Objective To investigate the expression of hsa_circ_0010697 in gastric cancer and its possible molecular mechanism in the development of gastric cancer.Methods The gene sequence of hsa_circ_0010697 was retrieved from the National Center for Biotechnology Information database,a lentiviral vector was synthesized,and gastric cancer cell line SGC-7901 was prepared.The cells were divided into 3 groups:control group(gastric cancer cell line SGC-7901 was not transfected with lentivirus vector),shRNA-NC group(gastric cancer cell line SGC-7901 was transfected with empty lentiviral vector),and hsa_circ_0010697-shRNA group(hsa_circ_0010697 lentivirus vector was transfected into gastric cancer cell line SGC-7901).qRT-PCR was used to detect the expression of hsa_circ_0010697 in gastric cancer cell line SGC-7901.The CCK-8 method was used to detect the cell proliferation(absorbance value at a wavelength of 450 nm,A450).The Tranwell experiment was used to detect the migration and invasion of cells(the number of cells penetrating the matrix membrane to reach the lower chamber).Western blot was used to detect the relative expression of RAS,extracelluar regulated protein kinases(ERK)1/2,cyclin-dependent kinase 4(CDK4),Cyclin D1,and recombinant E2F transcription factor 1(E2F1)proteins in the RAS-ERK signaling pathway.Six 6-week-old male SPF nude mice were randomly divided into the shRNA-NC group and hsa_circ_0010697-shRNA group,with three mice in each group.The body weight and tumor volume of nude mice were measured.Results The relative expression level of genes in the hsa_circ_0010697 shRNA group was 1.423±0.109,which was significantly higher than that in the control group and shRNA-NC group(0.994±0.172,1.006±0.137,both P＜0.05).The cell A450 value of the hsa_circ_0010697-shRNA group was 0.757±0.051,which was significantly lower than that in the control group and shRNA-NC group(1.000±0.044,0.981±0.054,both P＜0.05).The number of cells penetrating the matrix membrane to reach the lower chamber in the hsa_circ_0010697-shRNA group was 93±11,which was significantly less than that in the control group and shRNA-NC group(165±16,152±15,both P＜0.05).The relative expression levels of RAS,ERK1/2,CDK4,Cyclin D1,and E2F1 proteins in the RAS-ERK signaling pathway in the hsa_circ_0010697 shRNA group were significantly higher than those in the control group and shRNA-NC group(all P＜0.05).On the day 12,18,22,and 24 of feeding,the body weight of nude mice in the hsa_circ_0010697 shRNA group was significantly higher than that in the shRNA-NC group(all P＜0.05).On the day 22 and 24 of feeding,the tumor volume of nude mice in the hsa_circ_0010697 shRNA group was significantly smaller than that in the shRNA-NC(all P＜0.05).Conclusion hsa_circ_0010697 can serve as a potential biomarker and therapeutic target in the diagnosis and treatment of gastric cancer,providing new ideas for targeted therapy for gastric cancer.
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