MALDI-TOF MS技术在临床微生物检验中的应用
Application of MALDI-TOF MS technology in clinical microbiological examination
段雪寒 1吴华1
作者信息
- 1. 海南省人民医院检验科,海南海口 570100
- 折叠
摘要
随着科学技术的发展,新技术在医学检验中的应用越来越广泛.相较于传统微生物鉴定繁琐、耗时的工作流程,基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS,简称MS)技术因其快速、灵敏、简便、省时和特异性高等特点,在菌种鉴定、耐药监测、突变菌株鉴别等方面发挥巨大的作用,逐渐成为临床微生物实验室不可或缺的微生物鉴定工具.虽然MS技术鉴定菌种高度依赖数据库,也在一定程度上受限于临床样本质量,但其在临床微生物检验中仍有较大的空间待开发.文章对MS技术在临床微生物检验中的应用进行综述.
Abstract
With the development of science and technology,the application of new technology in medical examination is more and more extensive.Compared with the complicated and time-consuming workflow of traditional microbial identification,matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS)has gradually become an indispensable microbial identification tool in clinical microbiological laboratories because of its rapid,sensitivity,simple,time-saving and high specificity.MALDI-TOF MS plays a role in isolate identification,drug resistance monitoring and mutation identification.Its identification relies on the database,and it is limited to a certain extent by the quality of clinical samples.MALDI-TOF MS will still have utilization space to be developed in clinical microbiological examination.
关键词
基质辅助激光解吸电离飞行时间质谱/快速鉴定/数据库/耐药监测Key words
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry/Rapid identification/Database/Drug resistance monitoring引用本文复制引用
基金项目
海南省自然科学基金面上项目(821MS130)
海南省临床医学中心建设项目()
出版年
2024
NETL
NSTL
万方数据