摘要
目的 探讨乳脂球表皮生长因子8(MFG-E8)和G蛋白亚基α-14(GNA14)在子宫内膜异位症相关性卵巢癌(EAOC)中的表达及其临床意义.方法 选取2015年5月—2017年5月华北医疗健康集团峰峰总医院EAOC患者51例(EAOC组)、非典型子宫内膜异位症(AEM)患者63例(AEM组)、卵巢型子宫内膜异位症(EMT)患者82例(EMT组).收集所有患者的临床资料,并检测病变组织中MFG-E8、GNA14表达.对EAOC患者随访5年.采用Kaplan-Meier生存曲线分析EAOC患者的生存情况.采用多因素Cox回归分析评估EAOC患者死亡的危险因素.结果 EAOC组MFG-E8阳性率为82.35％、GNA14阳性率为70.59％,均显著高于 AEM 组(41.27％、44.44％)和 EMT 组(25.61％、23.17％)(P＜0.001).MFG-E8 高表达组和低表达组肿瘤直径、肿瘤级别、国际妇产科联合会(FIGO)分期、淋巴转移情况差异均有统计学意义(P＜0.05).GNA14高表达组和低表达组淋巴转移情况差异有统计学意义(P=0.009).Kaplan-Meier生存曲线分析结果显示,MFG-E8高表达组和GNA14高表达组无进展生存期分别短于MFG-E8低表达组和GNA14低表达组(P＜0.05).多因素Cox回归分析结果显示,FIGO分期Ⅱ期、淋巴转移、MFG-E8高表达、GNA14高表达是EAOC患者死亡的独立危险因素[风险比(HR)值分别为2.337、2.519、3.133、3.080,95％可信区间(CI)分别为 1.258～4.342、1.332～4.764、1.381～7.108、1.318～7.197,P＜0.01].结论 MFG-E8、GNA14在EAOC患者癌组织中呈高表达,且与患者预后不良密切相关,或可作为EAOC患者的预后评估指标.
Abstract
Objective To investigate the expressions of milk fat globule-epidermal growth factor-8(MFG-E8)and G protein subunit alpha 14(GNA14)in endometriosis associated ovarian cancer(EAOC).Methods A total of 51 patients with EAOC(EAOC group),63 patients with atypical endometriosis(AEM)(AEM group)and 82 patients with ovarian endometriosis(EMT)(EMT group)were enrolled from Fengfeng General Hospital of North China Medical and Health Group from May 2015 to May 2017.The clinical data of all the patients were collected,and the expressions of MFG-E8 and GNA14 in pathological tissues were determined.The EAOC patients were followed up for 5 years.Kaplan-Meier survival curve was used to analyze the survival of EAOC patients.Multivariate Cox regression analysis was used to evaluate the risk factors of death in EAOC patients.Results The positive rates of MFG-E8 and GNA14 in EAOC group were 82.35％and 70.59％,respectively,which were higher than those in AEM group(41.27％,44.44％)and EMT group(25.61％,23.17％)(P＜0.001).There was statistical significance in tumor diameter,tumor grade,the International Federation of Gynecology and Obstetrics(FIGO)stage and lymphatic metastasis between MFG-E8 high expression group and low expression group(P＜0.05).There was statistical significance in lymphatic metastasis between GNA14 high expression group and low expression group(P=0.009).The progression-free survival times of MFG-E8 high expression group and GNA14 high expression group were shorter than those of MFG-E8 low expression group and GNA14 low expression group,respectively(P＜0.05).FIGO stage Ⅱ,lymphatic metastasis,MFG-E8 high expression and GNA14 high expression were independent risk factors for death in EAOC patients[hazard ratios(HR)were 2.337,2.519,3.133 and 3.080,95％confidence intervals(CI)were 1.258-4.342,1.332-4.764,1.381-7.108 and 1.318-7.197,respectively,P＜0.01].Conclusions MFG-E8 and GNA14 are highly expressed in cancer tissues of EAOC patients,and they are related to poor prognosis of patients,which may be used as prognostic indicators of EAOC patients.
基金项目
国家自然科学基金项目(81473268)
河南省医学科技攻关计划联合共建项目(LHGJ20190358)
NETL
NSTL
万方数据