CRISPR/Cas核酸检测系统的研究进展和未来挑战
Research progress and challenges on CRISPR/Cas nucleic acid diagnosis system
周建 1任雪梅 1王馨 1李卓1
作者信息
- 1. 西安医学院第一附属医院检验科,陕西西安 710077
- 折叠
摘要
成簇规律间隔短回文重复序列(CRISPR)/成簇规律间隔短回文重复序列相关蛋白(Cas)系统是一种新兴的分子诊断技术,具有检测速度快、操作简单、特异性高等优势,目前已成为分子诊断领域的研究热点.基于CRISPR/Cas系统构建的核酸检测系统中有一部分已得到临床验证,并获得预期的结果,有望成为一种理想的核酸分子诊断技术,但其在临床大规模应用前尚有一系列问题需要解决.文章对已建立的CRISPR/Cas核酸检测系统的原理、特点和存在的问题进行综述,以期为CRISPR/Cas核酸检测系统的临床应用提供依据.
Abstract
Clustered regularly interspaced short palindromic repeat(CRISPR)/clustered regularly interspaced short palindromic repeat-associated protein(Cas)nucleic acid diagnosis system,as a new molecular diagnosis technology has advantages of rapid determination,simple operation and high specificity.CRISPR/Cas has been a research hotspot in nucleic acid molecular diagnosis.Some CRISPR/Cas nucleic acid diagnosis systems have been validated by clinical samples,which are expected to be an ideal nucleic acid molecular diagnosis technology.However,there are still a series of problems to be solved before the clinical large-scale commercial application.In this review,the principle,characteristics and problems are summarized,and it is expected to provide a reference for the early clinical application of CRISPR/Cas nucleic acid diagnosis system.
关键词
成簇规律间隔短回文重复序列/成簇规律间隔短回文重复序列相关蛋白/分子诊断技术/核酸Key words
Clustered regularly interspaced short palindromic repeat/Clustered regularly interspaced short palindromic repeat-associated protein/Molecular diagnosis technology/Nucleic acid引用本文复制引用
基金项目
陕西省卫生健康委联合攻关项目(B类)(2022B009)
西安市科技局西安市创新能力强基计划-医学研究项目(22YXYJ0126)
西安医学院校级科技创新团队项目(2021TD14)
出版年
2024
NETL
NSTL
万方数据