Objective To evaluate the recognition ability and the accuracy of quantitative determination results of 6 commonly used clinical glycated hemoglobin A1c(HbA1c)determination systems for hemoglobin(Hb)variants.Methods A total of 245 suspected Hb variant interference samples were collected from HbA1c routine test in Shanghai Jiahui International Hospital and the Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from December 2020 to December 2022.Six commonly used clinical determination systems(5 systems were high performance liquid chromatography,and 1 system was capillary electrophoresis)were used to determine suspected interference samples,and the types of Hb variants were confirmed by sequencing method.Based on the results of cobas c513 automatic glycated hemoglobin analyzer,the accuracy of 6 determination systems was evaluated.Results Totally,245 suspected Hb variant interference samples were verified by sequencing,141 Hb variant interference samples were determined.Eleven Hb variants(Hb G-Coushatta,Hb G-Taipei,Hb J-Bangkok,Hb D-Los Angeles,Hb Pyrgos,Hb Q-Thailand,Hb Queens,Hb Quong Sze,Hb C,Hb E,Hb S)were determined.Only Hb D-Los Angeles and Hb S can be determined by all the 6 determination systems,and the determination systems for the other 9 Hb variants had different recognition abilities.Except for Hb S,the results of 6 determination systems for the interference samples of other 10 Hb variants were different from those of cobas c513.Conclusions The commonly used clinical HbA1c determination system is not able to identify most of the Hb variants.For the interference of Hb variants,different methods can be used for determination and sequencing verification if necessary.
维普
万方数据
