Study on anti-inflammatory effect of components absorbed into blood of aqueous extract of"Curculiginis Rhizoma and Epimedii Folium"
Objective:To explore the anti-inflammatory effect of the components absorbed into serum of the aqueous extract of"Curculiginis Rhizoma and Epimedii Folium"(EX).Methods:AutoDockTools software was used to dock the five components absorbed into serum of EX(Curculigoside,Icariin,Baohuoside I,Oricinol glucoside and Epimedin A)with target protein TREM2.CCK8 method was used to detect the effects of the five components on BV2 cell viability at different concentrations.LPS was used to stimulate BV2 or mouse bone marrow-derived BMDMs cells and Nigericin to stimulate LPS pretreated BMDMs cells to establish inflammatory models,and the effects of the five components on TNF-α and IL-1β levels in the cell supernatant were detected by ELISA.Results:The components absorbed into serum of EX including Curculigoside,Icariin,Baohuoside I and Epimedin A,bound very stably to TREM2,while the binding of Orcinol glucoside to TREM2 was relatively stable.Curculigoside,Icariin,Baohuoside I,Orcinol glucoside and Epimedin A all reduced the level of IL-1 β produced by BMDMs at certain concentrations,while only high concentrations of Curculigoside and Icariin reduced the level of TNF-α produced by BMDMs.Conclusion:5 components absorbed into serum of EX could all bind to TREM2 and inhibit downstream NF-κB or NLRP3 inflammatory pathways to improve inflammation,which might be an effective material basis for EX to regulate TREM2 to improve learning and memory ability and neuroinflammation,and work synergistically in EX.
Curculiginis Rhizoma and Epimedii Foliumaqueous extractTREM2molecular dockinginflammation
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