Effects of Jianpi Xiaoying Formula on Th1 and Th17 in mice of Hashimoto's thyroiditis model
Objective:To investigate whether Jianpi Xiaoying Formula improving Hashimoto's thyroiditis(HT)by regulating the proportion of helper T cells(Th).Methods:Forty female CBA/J mice were randomly divided into five groups:the normal control group,model control group,and the low,medium and high dose groups of Jianpi Xiaoying Formula,eight mice in each group.Except for the normal control group,the HT model was induced by subcutaneous injection of porcine thyroglobulin combined with iodized water feeding in mice of other groups.At the end of modeling,the mice in the normal control group and model control group were given 0.5 mL of drinking water by gavage every day,and the mice in the low,medium and high dose groups of Jianpi Xiaoying Formula were given aqueous solution of Jianpi Xiaoying Formula granules(with the doses of 8.632 g/kg.17.264 g/kg and 34.528 g/kg respectively)by gavage every day,for consecutive 6 weeks.At the end of intervention,the levels of serum thyroid peroxidase antibody(TPOAb),thyroglobulin antibody(TGAb),free triiodothyronine(FT3),free thyroxine(FT4)and thyrotropin(TSH)in mice were detected by enzyme-linked immunosorbent assay(ELISA);the proportions of Thl.Th2,Th17 and regulatory T cell(Treg)in peripheral blood of mice were detected by flow cytometry;the gene expressions of tumor necrosis factor-α(TNF-α),γ interferon(IFN-γ),interleukin-17(IL-17),T-bet and RORγ-t were detected by real-time fluorescence quantitative PCR;the protein expressions of T-bet and RORγ-t in spleen tissues were detected by Western blot;the pathological and morphological changes of thyroid tissue were observed by hematoxylin-eosin(HE)staining.Results:①Compared with the model control group,the level of serum TPOAb of mice in the high dose group of Jianpi Xiaoying Formula was significantly decreased(P＜0.05),and the level of TGAb of mice in the low and high dose groups of Jianpi Xiaoying Formula was also significantly decreased(P＜0.05).②Compared with the model control group,the serum FT3 and FT4 levels of mice in the high dose group of Jianpi Xiaoying Formula were significantly increased(P＜0.05).③ Jianpi Xiaoying Formula can improve the structure and colloid of thyroid follicles,make the follicle morphology regular,and reduce the degree of infiltration of inter-follicular lymphocytes and plasma cells in thyroid of the HT model mice.④Compared with the model control group,the Th1 ratio in peripheral blood of mice in the low,medium and high dose groups of Jianpi Xiaoying Formula was significantly decreased(P＜0.05),and the Th17 ratio of mice in the low and high dose groups of Jianpi Xiaoying Formula was also significantly decreased(P＜0.05).⑤Compared with the model control group,the relative expressions of T-bet and RORγ-t genes in the spleen tissues of mice in the low,medium and high dose groups of Jianpi Xiaoying Formula were significantly decreased(P＜0.05),the protein expressions of T-bet and RORγ-t in the high dose group of Jianpi Xiaoying Formula were significantly decreased(P＜0.05),and the protein expression of T-bet in the medium dose group of Jianpi Xiaoying Formula was significantly decreased(P＜0.05).⑥Compared with the model control group,the relative expressions of TNF-α,IFN-γ and IL-17 genes of mice in each dose groups of Jianpi Xiaoying Formula were significantly decreased(P＜0.05).Conclusion:Jianpi Xiaoying Formula can inhibit the inflammatory response by down-regulating the transcription and differentiation of Th1 and Th17 and the secretion of their related inflammatory factors,thus improve HT.
Hashimoto's thyroiditisJianpi Xiaoying Formulahelper T cell 1helper T cell 17
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