Therapeutic effect of Jianpi Huazhi Formula on acute ulcerative colitis mice and its influence on inflammatory factors
Objective:To study the therapeutic effect of Jianpi Huazhi Formula(JHF)on acute ulcerative colitis(UC)model mice,and explore its mechanism of action based on the expression levels of inflammatory factors in serum and colon tissue of mice.Methods:Seventy-six healthy male C57 mice were randomly divided into normal group,model group,mesalazine group,and low,medium,and high dose JHF groups.An acute UC mouse model was established by drinking 3％dextran sulfate sodium(DSS)solution.The low,medium,and high dose JHF groups were orally administered with 145.8,291.5,583.0 g/L JHF solution respectively,while the mesalazine group was orally administered with 10 g/L mesalazine solution.The symptoms of mice in each group were observed,the disease activity index(DAI)was scored,and the pathological changes of colon tissue were observed by hematoxylin-eosin(HE)staining.Serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),direct bilirubin(DBil),total bilirubin(TBil),albumin(ALB),alkaline phosphatase(ALP),γ-glutamyl transpeptidase(γ-GT),total bile acid(TBA),creatinine(Cre),uric acid(UA)and blood urea nitrogen(BUN)were measured by rate method and endpoint method.The levels of interleukin(IL)-6,tumor necrosis factor-α(TNF-α)and IL-10 in serum and colon tissue of mice were determined by enzyme-linked immunosorbent assay(ELISA).Results:JHF could effectively improve clinical symptoms such as bloody stools and diarrhea in acute UC mice,improve body mass loss and colon length reduction of mice,reduce DAI score of mice(P＜0.05),alleviate the degree of pathological inflammation damage in colon tissue,reduce the expression levels of pro-inflammatory cytokines IL-6 and TNF-α,and increase the expression level of anti-inflammatory factor IL-10(P＜0.05,P＜0.01).Conclusion:JHF has a good therapeutic effect on acute UC mice,and its mechanism of action may be to repair local damage to the colon mucosa of UC mice,regulate the levels of inflammatory factors in serum and colon tissue.
NETL
NSTL
万方数据
