Objective:To establish the quality standard of Dracocephalum moldavica in different areas of Inner Mongolia,and to provide experimental basis for the quality control of this herb.Methods:Dracocephalum moldavica were collected from different areas of Inner Mongolia during the summer blooming period,dried.The morphological features,microscopic features,thin-layer chromatography(TLC)identification methods of Dracocephalum moldavica,and high performance liquid chromatography(HPLC)method for the determination of rosmarinic acid content were established.According to the relevant methods in the general rules of Pharmacopoeia of the People's Republic of China(2020 Edition),the moisture,total ash,acid-insoluble ash and extracts content of Dracocephalum moldavica were determined.Results:The characteristics,microscopic characteristics and TLC of Dracocephalum moldavica had obvious exclusive characteristics.The moisture content was 6.20％～8.42％.The total ash content was 6.79％～12.11％.The acid-insoluble ash content was 0.71％～4.28％.The water-soluble extracts content by hot immersion method was 23.45％～35.85％,and the water-soluble extracts content by cold immersion method was 15.61％～32.74％.The concentration of rosmarinic acid showed a good linear relationship in the range of 0.30～1.80 mg/L(r=0.999 8).The average recovery rate was 97.89％and the R.SD was(0.25～1.68)％.The content of rosmarinic acid in 9 batches of Dracocephalum moldavica samples ranged from 0.94 to 7.25 mg/g,with an average content of 4.27 mg/g.Conclusion:The established method is simple,accurate and reproducible,and can effectively evaluate the quality of Dracocephalum moldavica,so as to ensure its effectiveness of clinical application in Mongolian medicine.
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