首页|蒙药材香青兰的质量标准研究

蒙药材香青兰的质量标准研究

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目的:建立内蒙古不同地区香青兰质量标准,为该药材质量控制提供实验依据.方法:夏季盛花期采集内蒙古不同地区香青兰,阴干;建立香青兰的性状、显微特征、薄层色谱(TLC)鉴别方法,以及迷迭香酸含量的高效液相色谱(HPLC)测定方法;按照《中华人民共和国药典(2020年版)》通则中的相关方法,对香青兰药材的水分、总灰分、酸不溶性灰分及浸出物等进行测定.结果:香青兰的性状、显微特征、TLC等均有明显的专属特征,水分为6.20％～8.42％,总灰分为6.79％～12.11％,酸不溶性灰分为0.71％～4.28％,热浸法水溶性浸出物为23.45％～35.85％,冷浸法水溶性浸出物为15.61％～32.74％.迷迭香酸浓度在0.30～1.80 mg/L(r=0.999 8)范围内呈良好线性关系,平均加样回收率为97.89％,RSD为(0.25～1.68)％.9批香青兰药材样品中迷迭香酸含量在0.94～7.25 mg/g,平均含量为4.27 mg/go结论:所建立的香青兰含量测定方法简便、准确、重复性好,能有效评价香青兰的质量,以保障其蒙医临床应用的有效性.
Study on quality standards of mongolian medicine Dracocephalum moldavica
Objective:To establish the quality standard of Dracocephalum moldavica in different areas of Inner Mongolia,and to provide experimental basis for the quality control of this herb.Methods:Dracocephalum moldavica were collected from different areas of Inner Mongolia during the summer blooming period,dried.The morphological features,microscopic features,thin-layer chromatography(TLC)identification methods of Dracocephalum moldavica,and high performance liquid chromatography(HPLC)method for the determination of rosmarinic acid content were established.According to the relevant methods in the general rules of Pharmacopoeia of the People's Republic of China(2020 Edition),the moisture,total ash,acid-insoluble ash and extracts content of Dracocephalum moldavica were determined.Results:The characteristics,microscopic characteristics and TLC of Dracocephalum moldavica had obvious exclusive characteristics.The moisture content was 6.20％～8.42％.The total ash content was 6.79％～12.11％.The acid-insoluble ash content was 0.71％～4.28％.The water-soluble extracts content by hot immersion method was 23.45％～35.85％,and the water-soluble extracts content by cold immersion method was 15.61％～32.74％.The concentration of rosmarinic acid showed a good linear relationship in the range of 0.30～1.80 mg/L(r=0.999 8).The average recovery rate was 97.89％and the R.SD was(0.25～1.68)％.The content of rosmarinic acid in 9 batches of Dracocephalum moldavica samples ranged from 0.94 to 7.25 mg/g,with an average content of 4.27 mg/g.Conclusion:The established method is simple,accurate and reproducible,and can effectively evaluate the quality of Dracocephalum moldavica,so as to ensure its effectiveness of clinical application in Mongolian medicine.

Dracocephalum moldavicaMongolian medicinal materialrosmarinic acidmicroscopic identificationthin-layer chromatographyhigh performance liquid chromatographyquality standard

莲花、呼日乐巴根、戴丽丽、白文明、渠弼、包勒朝鲁、那生桑

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内蒙古医科大学蒙医药学院(内蒙古 呼和浩特 010110)

内蒙古医科大学药学院(内蒙古 呼和浩特 010110)

内蒙古医科大学民族医药创新中心(内蒙古 呼和浩特 010110)

香青兰 蒙药材 迷迭香酸 显微鉴别 薄层色谱 高效液相色谱 质量标准

内蒙古自治区蒙医药标准化项目内蒙古自治区蒙医药标准化项目内蒙古医科大学科技创新团队建设项目内蒙古医科大学面上项目

2023-[MB027]MB2019-02RZ2200002685YKD2023MS062

2024

10.16306/j.1008-861x.2024.03.004

上海中医药大学学报
上海中医药大学,上海市中医药研究院

上海中医药大学学报

CSTPCD
影响因子：0.788
ISSN：1008-861X
年,卷(期)：2024.38(3)
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