云南藤黄提取物对结直肠癌细胞侵袭转移的抑制作用
Mechanism of Garcinia Yunnanensis Extract effecting on invasion and metastasis of colorectal cancer cell
柴琼 1张璐 2李波旺 3巩伟伟 4段琳琳 1隋华5
作者信息
- 1. 上海中医药大学中药学院(上海 201203)
- 2. 郑州大学附属肿瘤医院(河南省肿瘤医院)中西医结合科(河南 郑州 450003)
- 3. 河南中医药大学研究生院(河南 郑州 450046)
- 4. 上海市第一康复医院代谢与肾病康复科(上海 200090)
- 5. 上海市第一人民医院嘉定医院实验医学中心(上海 201803)
- 折叠
摘要
目的:探讨云南藤黄乙醇标准提取物(YTE-17)抑制结直肠癌转移的作用效果及其潜在机制.方法:采用克隆形成实验观察YTE-17 对结直肠癌细胞HCT116 和LoVo的生长和增殖情况;划痕和Transwell实验检测YTE-17对结直肠癌侵袭迁移能力的影响;蛋白质免疫印迹法(Western blot)检测YTE-17 对基质金属蛋白酶-2(MMP-2)、生存素(Survivin)、细胞周期蛋白D1(cyclin D1)、E-钙黏着蛋白(E-cadherin)和波形蛋白(Vimentin)表达的调节作用.结果:YTE-17 可显著抑制结直肠癌细胞HCT116 和LoVo的生长和增殖;与对照组(YTE-17 干预 0 μg/mL)比较,YTE-17 能够抑制HCT116 和LoVo细胞侵袭迁移能力(P<0.01),且呈浓度依赖性.Western blot 结果显示,YTE-17可以降低HCT116 细胞中Survivin、Cyclin D1 和E-cadherin的表达,并呈剂量依赖性(P<0.01);YTE-17 可以降低LoVo细胞中Survivin、Cyclin D1、MMP-2 和Vimentin的表达,并呈剂量依赖性(P<0.01).结论:YTE-17 可通过降低Survivin、Cyclin D1、MMP-2、E-cadherin和Vimentin表达抑制结直肠癌的侵袭转移.
Abstract
Objective:To explore the inhibitory effects and its potential mechanisms of YTE-17,a standard ethanolic extract of Garcinia Yunnanensis,on the metastasis of colorectal cancer.Methods:Clone formation assay was used to observe the growth and proliferation of colorectal cancer cells HCT116 and LoVo treated with YTE-17.Scratch test and Transwell assay were performed to detect the effect of YTE-17 on the invasion and migration ability of colorectal cancer.Western blot analysis was conducted to detect the regulatory effect of YTE-17 on the expressions of MMP-2,Survivin,Cyclin D1,E-cadherin,and Vimentin.Results:YTE-17 significantly inhibited the growth and proliferation of colorectal cancer cells HCT116 and LoVo.Compared with the control group,YTE-17 suppressed the invasion and migration abilities of HCT116 and LoVo cells(P<0.01)in a dose-dependent manner.Western blot results revealed that YTE-17 reduced the expressions of Survivin,Cyclin D1 and E-cadherin proteins in HCT116 cells in a dose-dependent manner(P<0.01),and reduced the expressions of Survivin,Cyclin D1,MMP-2 and Vimentin proteins in LoVo cells in a dose-dependent manner(P<0.01).Conclusion:YTE-17 can inhibit the invasion and metastasis of colorectal cancer by reducing the expressions of Survivin,Cyclin D1,MMP-2,E-cadherin and Vimentin proteins.
关键词
结直肠癌/云南藤黄/侵袭转移/Survivin/Cyclin/D1/MMP-2/E-cadherin/VimentinKey words
colorectal cancer/Garcinia Yunnanensis/invasion and metastasis/Survivin/Cyclin D1/MMP-2/E-cadherin/Vimentin引用本文复制引用
出版年
2024
NETL
NSTL
万方数据