Mechanism of Garcinia Yunnanensis Extract effecting on invasion and metastasis of colorectal cancer cell
Objective:To explore the inhibitory effects and its potential mechanisms of YTE-17,a standard ethanolic extract of Garcinia Yunnanensis,on the metastasis of colorectal cancer.Methods:Clone formation assay was used to observe the growth and proliferation of colorectal cancer cells HCT116 and LoVo treated with YTE-17.Scratch test and Transwell assay were performed to detect the effect of YTE-17 on the invasion and migration ability of colorectal cancer.Western blot analysis was conducted to detect the regulatory effect of YTE-17 on the expressions of MMP-2,Survivin,Cyclin D1,E-cadherin,and Vimentin.Results:YTE-17 significantly inhibited the growth and proliferation of colorectal cancer cells HCT116 and LoVo.Compared with the control group,YTE-17 suppressed the invasion and migration abilities of HCT116 and LoVo cells(P<0.01)in a dose-dependent manner.Western blot results revealed that YTE-17 reduced the expressions of Survivin,Cyclin D1 and E-cadherin proteins in HCT116 cells in a dose-dependent manner(P<0.01),and reduced the expressions of Survivin,Cyclin D1,MMP-2 and Vimentin proteins in LoVo cells in a dose-dependent manner(P<0.01).Conclusion:YTE-17 can inhibit the invasion and metastasis of colorectal cancer by reducing the expressions of Survivin,Cyclin D1,MMP-2,E-cadherin and Vimentin proteins.
colorectal cancerGarcinia Yunnanensisinvasion and metastasisSurvivinCyclin D1MMP-2E-cadherinVimentin
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