Separation and determination of stilbenes ingredients in Dendrobii Officialis Caulis
Objective To separate chemical indicator and establish a HPLC method for determining the indicator in herbs from Dendrobii Offwialis Caulis.Methods The constituents were separated and purified by column chromatography on silica gel and Sephadex LH-20,as well as the chemical structures were determined by spectral data analyses.Determination of content of 3,4-dihydroxy-5,4'-dimethoxy bibenzyl (DDB-1) was performed by using high performance liquid chromatography.A Phenomenex-C18 column (250 mm × 4.60 mm,5 μm) was adopted with a mobile phase of acetonitrile-water,the runs were set at 230 nm.Results Four stilbenoids compounds were isolated and identified as 3,4-dihydroxy-5,4'-dimethoxybibenzyl,3,4-dihydroxy-5-methoxybibenzyl,5-hydroxy-3,4'-dimethoxybibenzyl,4'-hydroxy-3 ',5'-dimethoxybibenzyl.DDB-1 was selected as an indicator to assess the quality of Dendrobii Officialis Caulis.This indicator showed a good linear relationship within the range of 0.044 ～ 0.44 μg (R2 =0.999 9).The determination of DDB-1 in tested samples from different production base was most different,and it had little difference between each other if Dendrobii Officialis Caulis harvested in different month.Conclusion This proposed method was found to be accurate,reliable and simple,providing rapid and special determination of DDB-1,which could be used to control quality of Dendrobii Officialis Caulis.
NETL
NSTL
万方数据
维普
