泽泻降低2型糖尿病小鼠血糖的有效部位筛选
Screening research on effective parts of Alisma orientalis (Samuel.) Juzep.associated with blood glucose decrease in type 2 diabetic mice
张伟云 1陈全成 2王丽荣 1温忠秀1
作者信息
- 1. 厦门医学高等专科学校中心实验室、厦门市中药生物工程重点实验室、福建省中药精加工与健康产品开发重点研究室、福建省道地药材生物工程重点实验室,厦门医学高等专科学校药学系 福建厦门361008
- 2. 厦门大学药学院 福建厦门361102
- 折叠
摘要
目的 初步探索泽泻[Alisma orientalis(Samuel.)Juzep.]降低2型糖尿病小鼠血糖的有效部位.方法 采用超声提取法获得泽泻乙醇提取物,再分别用石油醚、乙酸乙酯、水饱和正丁醇和水相进行萃取分离,得到的泽泻乙醇提取物及其各溶剂萃取物应用于腹腔注射烟碱、链脲霉素诱导的2型糖尿病小鼠模型.造模成功后,将动物分组,其中空白对照组(未注射链脲霉素和烟碱的小鼠)和2型糖尿病模型组小鼠连续灌胃0.9％ NaC1溶液21 d(灌胃剂量为10 μl/g),测试药物组分别连续灌胃泽泻乙醇提取物、泽泻石油醚萃取物、泽泻乙酸乙酯萃取物、泽泻正丁醇萃取物、泽泻水萃取物21 d(各测试药物灌胃高、中、低剂量分别为100μg/g、50μg/g、25 μg/g),阳性对照组连续灌胃罗格列酮溶液21 d(灌胃剂量为10 μg/g),每组8只小鼠,筛选具有降低2型糖尿病小鼠血糖的泽泻有效部位.用具有降低2型糖尿病小鼠血糖的有效部位处理3T3-L1前脂肪细胞,观察其对前脂肪细胞分化过程的影响.结果 与2型糖尿病模型组比较,连续灌胃泽泻乙酸乙酯萃取物21 d(剂量为100 μg/g)降低了2型糖尿病小鼠的血糖,改善了口服葡萄糖耐受试验(oral glucose tolerance test,OGTT)过程中的糖耐量(P＜0.01).1 mg/L和10 mg/L的泽泻乙酸乙酯萃取层均能促进前脂肪细胞的分化过程.结论 泽泻乙酸乙酯萃取物降低了2型糖尿病小鼠血糖,促进了3T3-L1前脂肪细胞分化,但其具体作用机制尚需深入研究.
Abstract
Objective To explore the effective parts of Alisma orientalis (Samuel.) Juzep.associated with blood glucose decrease in type 2 diabetic mice.Methods Ethanol extracts from A.orientalis were obtained by ultrasonic extraction.Then,the ethanol extract was extracted and separated with petroleum ether,ethyl acetate,water saturated n-butanol and water respectively.After decompressing concentration,these solvent extractions were applied to type 2 diabetic mice model induced by intraperitoneal injection of streptozotocin and nicotine.After successful modeling,the mice were divided into the blank control group (without injection of streptozotocin and nicotine),model group of type 2 diabetes mellitus,positive control group and test drug groups,8 in each group.The blank control group and model group were treated with 0.9％ NaCl at the dose of 10μ l/g,the test drug groups were treated with corresponding extracts from different solvent extractions described above at the high-,mid-,low-dose of 100,50,25 μg/g respectively.The positive control group was treated with rosiglitazone at the dose of 10μg/g.The treatment course was 21 days for intragastric administration.The effective parts of Alisma orientalis (Samuel.) Juzep.associated with blood glucose decrease in type 2 diabetic mice were screened and then the effect of the screened effective part on the differentiation of 3T3-L1 pre-adipocyte was observed.Results Compared with the model group,the blood glucose was decreased after the intragastric administration with ethyl acetate extract of A.orientalis at dose of 100μ g/g for 21 days and the glucose tolerance during the oral glucose tolerance test (OGTT) was improved(P ＜0.01).The differentiation of pre-adipocyte was promoted when the ethyl acetate extract of A.orientalis at the concentration of 1 mg/L and 10 mg/L.Conclusion The ethyl acetate extract of A.orientalis reduced the blood glucose in type 2 diabetic mice and promoted the differentiation of 3T3-L1 pre-adipocytes,but the specific mechanism still need to be further studied.
关键词
泽泻/2型糖尿病小鼠/血糖/3T3-L1前脂肪细胞分化Key words
Alisma orientalis (Samuel.) Juzep./type 2 diabetic mice/blood glucose/differentiation of 3T3-L1 pre-adipocytes引用本文复制引用
基金项目
福建省科技厅自然科学基金青年创新项目()
厦门市卫计委资助项目(2014D008)
福建省卫生系统中青年骨干人才培养项目基础项目()
厦门市卫计委资助项目(2015-ZQN-JC-45)
福建省科技厅自然科学基金(2015J01065)
福建省教育厅中青年教师教育科研项目杰青项目(JA14421)
福建省教育厅中青年教师教育科研项目(JA13425)
厦门市科技局科技计划高校创新项目(3502Z20143026)
厦门市科技局科技计划项目(3502Z20144031)
厦门医学高等专科学校科研基金(Z2013-12)
厦门医学高等专科学校科研基金(Z2013-25)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据