Molecular Identification of Codonopsis Radix and Its Adulterants Based on ITS2 Barcode
This study aimed to discriminate between Codonopsis Radix and its adulterants by using ITS2 barcode sequence. DNA was extracted from Codonopsis Radix specimens. The ITS2 barcode was amplified by PCR and sequenced bidirectionally. Sequence assembly and generation of consensus sequence were conducted by using the CodonCode Aligner. The interspecific divergence and intraspecific variation of 'and its adulterants were computed.And then N-J tree was constructed by MEGA V4. 1. The ITS2 secondary structures were predicted by database which was established by Koetschan et al. Results showed that the average interspecific K2P distance of Codonopsis Radix was 0.2688, while the intraspecific was 0.0339. N-J tree revealed that different sources of Codonopsis Radix clustered and its adulterants were obviously distinguished. Codonopsis Radix was differentiated from its adulterants in helix I and helix III of the ITS2 secondary structure. It is concluded that ITS2 barcode sequence can be used to distinguish Codonopsis Radix from its adulterants.
NETL
NSTL
万方数据
维普
