A Research on miRNA-34b Expression Regulated by Ophiopogonin-B in Vitro in Non-Small Cell Lung Cancer A549 Cells
This study aimed to observe miRNA-34b expression through different treatments of non-small cell lung cancer (NSCLC),and investigate the regulatory effect of miR-34b on MET signaling pathway and the molecular mechanism of anti-tumor effect of ophiopogonin-B (OPB),and explore the new target for the treatment of NSCLC.Taking A549 cells,MRC-5 cells,A549 cells with OPB treatment,A549 cells infected with hsa-miR-34b-over-expressed lentivirus vector,and A549 cells infected with negative control CON181B virus as research objects,the CON group,the MRC-5 group,the CON+OPB group,the Micro-up group and the NC group were involved in the study.The expression of miR-34b in the A549 group,the MRC-5 group and the A549+OPB group were detected by qRT-PCR.Downstream targets of miR-34b were predicted by a bioinformatics software.Quantitative analysis was performed to quantify the expression of MET gene by qRT-PCR method with OPB administration.The effects of miR-34b and OPB on the expression of MET protein was tested by Western blot.Compared with the MRC-5 group,the expression of miR-34b in A549 cells was significantly decreased,but increased combining with OPB.TargetScan bioinformatics software showed that MET gene was prodicted to be the high-related downstream target gene of miR-34b.Within the administration of OPB,both genatic and protein levels of MET were decreased.In conclusion,OPB may present its anti-tumor effects through up-regulating miR-34b expression in A549 cells and supposing its downstream gene MET.
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万方数据
维普
