Study on the HPLC Fingerprints of the Ultrafine Granular Powder, the Ultrafine Powder and Decoction Pieces Made from Angelicae Sinensis Radix
This study aimed to establish an effective measurement to exam the quality of three formulations,ultrafine granular powder,ultrafine powder and decoction pieces made from Angelicae sinensis Radix through determining the content of ferulic acid by HPLC fingerprints.RP-HPLC method was performed on an Agilent ZORBAX SB column with a gradient elution using acetonitrile-l％ acetic acid at the flow rate of 1 mL·min-1.The column temperature was 30 ℃,and the detective wavelength was 254 nm.In addition,the contents of ferulic acid of the samples were detected according to Chinese Pharmacopoeia (2015 Edition).As a result,the fingerprints of the three formulations of Angelicae sinensis Radix were analyzed.The common pattern of chromatographic fingerprints in which fourteen mutual peaks were marked,was established with 30 batches of samples with 10 batches for each formulation of Angelicae sinensis Radix.The similarity among the fingerprints of the ultrafine granular powder,the granular powder and the decoction pieces were higher than 0.96.The content of ferulic acid in the ultrafine granular powder of Angelicae sinensis Radix was slightly higher than that in its decoction pieces.In conclusion,this method was reliable and stable and with good reproducibility,which was feasible in the area of drug quality control of ultrafine granular powder of Angelicae sinensis Radix.
NETL
NSTL
万方数据
维普
