首页|参仙升脉口服液化学成分的UHPLC-Q Exactive Focus MS/MS快速分析

参仙升脉口服液化学成分的UHPLC-Q Exactive Focus MS/MS快速分析

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
目的 探究参仙升脉口服液的化学物质基础。方法 利用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UHPLC-Q Exactive Focus MS/MS)对参仙升脉口服液的化学成分进行整体、系统辨识。采用Thermo Accucore aQ C18色谱柱(150 mm×2。1 mm，2。6 µm)，流动相0。1%甲酸水溶液(A)-甲醇(B)梯度洗脱，洗脱程序为0-13 min，5%-60%B;13-27 min，60%-95%B;27-30 min，95%B，流速0。3 mL·min-1，柱温30℃;质谱分析离子化方式为加热电喷雾离子化(H-ESI)，扫描模式为正、负离子切换，扫描范围m/z 120-1800，碰撞能量30 eV、50 eV、70 eV。结果 共鉴定出160个化学成分，包括29个黄酮类成分，24个有机酸类成分，21个生物碱类成分，19个萜类成分，15个苯丙素类成分，12个皂苷类成分和40个其他类成分;其中6个化学成分(芦丁、补骨脂苷、异补骨脂苷、补骨脂素、异补骨脂素和补骨脂酚)经对照品比对确认。结论 该研究借助UHPLC-Q Exactive Focus MS/MS技术实现了参仙升脉口服液化学成分准确、快速、系统的分析和鉴定，为其化学物质基础的阐明和质量控制提供了重要依据。
Rapid Analysis of Chemical Constituents in Shenxianshengmai Oral Liquid by UHPLC-Q Exactive Focus MS/MS
Objective To explore the chemical basis of Shenxianshengmai oral liquid.Method UHPLC-Q Exactive Focus MS/MS technology was used to identify the chemical components of Shenxianshengmai oral liquid.The chromatographic separation was performed on a Thermo Accucore aQ C18 column(150 mm×2.1 mm,2.6 µm)with mobile phase gradient elution of 0.1%formic acid aqueous solution(A)and methanol(B)for 0-13 min,5%-60%B;13-27 min,60%-95%B;27-30 min,95%B,the flow rate was 0.3 mL·min-1,and the column temperature was at 30℃.The mass spectrometry was performed by heating electrospray ionization(H-ESI)with positive and negative ion scanning modes.The scanning range was m/z 120-1800,and the collision energies were 30 eV,50 eV and 70 eV.Result A total of 160 components were identified,including 29 flavonoids,24 organic acids,21 alkaloids,19 terpenoids,15 phenylpropanoids,12 saponins and 40 other components.Six chemical constituents(rutin,psoralenoside,isopsoralenoside,psoralen,isopsoralen and bakuchiol)were confirmed by comparison with reference substances.Conclusion In this study,an UHPLC-Q Exactive Focus MS/MS method has been established for accurate,rapid and systematic identification of the constituents in Shenxian Shengmai oral liquid,which provides an important basis for clarifying the chemical basis and quality control.

Shenxianshengmai oral liqidUHPLC-Q Exactive Focus MS/MSChemical constituentsFlavonoidsOrganic acids

宗时宇、狄志彪、刘琪琪、黄壮壮、张红、李晔、刘峰、刘洋

展开 >

陕西省中医药研究院中药研究所 西安 710003

陕西中医药大学药学院 咸阳 712046

陕西步长制药有限公司 西安 710075

参仙升脉口服液 超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UHPLC-Q Exactive Focus MS/MS) 化学成分 黄酮 有机酸

陕西省自然科学基金委员会一般项目(青年)陕西省卫生计生委重点产业创新链(群)-社会发展领域项目陕西省卫生计生委创新人才推进计划青年科技新星项目

2020JQ-9872021ZDLSF04-062019KJXX-020

2024

10.11842/wst.20220110008

世界科学技术-中医药现代化
中科院科技政策与管理科学研究所，中国高技术产业发展促进会

世界科学技术-中医药现代化

CSTPCD北大核心
影响因子：1.175
ISSN：1674-3849
年,卷(期)：2024.26(1)
  • 48