Exploring the Effects of Lifei-Xiaoji Pills on Cycle,Proliferation and Migration of NSCLC A549 Cell Based on Hedgehog Pathway
Objective To investigate the effect of Lifei-Xiaoji pills on A549 cells and the regulation of Hedgehog pathway.Methods MTT assay was used to select the optimal intervention concentration of Lifei-Xiaoji containing serum on A549 cells.Then A549 cells were divided into 6 groups:Control group,Lifei-Xiaoji containing serum group,Hedgehog pathway inhibitor group(Vismodegib group),Cisplatin group,Lifei-Xiaoji containing serum + Vismodegib group,Lifei-Xiaoji containing serum + cisplatin group.After different interventions,cell proliferation ability was detect by CCK-8 assay,cell cloning into pellets was observed by clonal formation assay,migration ability was observed by transwell assay,the change of cell cycle ratio was observed by flow cytometry,mRNA and protein expressions of Gli1,Ptch1,Smo and CyclinD1 in Hedgehog pathway were detected by qRT-PCR and Western blot,respectively.Results The serum containing Lifei-Xiaoji pills with 10%concentration could significantly inhibit the proliferation of A549 cells(P<0.05),decrease cell clonogenesis and migration ability(P<0.05),block the cell cycle transition from G1 phase to S phase,inhibit Gli1,Ptch1,Smo and CyclinD1 mRNA and protein expression(P<0.05),and has good synergistic effect with Vismodegib.Conclusion Lifei-Xiaoji Pills can significantly inhibit A549 cell proliferation and migration,and the mechanism may be related to the down-regulation of Hedgehog pathway and cell cycle arrest.
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