Mechanism of Shuganheweitang in Improving Depression Rats by Integrating Intestinal Flora and Metabolomics Analysis
Objective To explore the antidepressant mechanism of action of SGHWT using 16S rDNA amplicon sequencing technology combined with non-targeted metabolomics techniques.Methods The chronic stress depression model was established by CUMS;To analyze the effect of SGHWT on the distribution of intestinal flora in depressed rats by 16S rDNA amplicon sequencing;To analyze the effect of SGHWT on differential metabolites and their metabolic pathways in hippocampal tissue of depressed rats by metabolomics technology.Combining Spearman analysis for correlation between differential intestinal flora and differential metabolites in hippocampal tissue.Results The results of behavioral experiments showed that,compared with the control group,the model group had significantly lower sugar-water preference rate,body weight and distance of open field exercise(P<0.01)and significantly longer forced swimming immobility time(P<0.01);Compared with the model group,SGHWT increased sugar water preference rate(P<0.01),increased body weight and distance of exercise(P<0.05,P<0.01)and shortened immobility time(P<0.01).The results of 16S rDNA amplicon sequencing revealed that SGHWT improved the abundance of Christensenellaceae_R-7_group,Kocuria,Oscillibacter,Colidextribacter,Intestinimonas,Subdoligranulum and Ruminococcus.A total of 66 differential metabolites were identified by non-targeted metabolomics analysis.The metabolic pathways involved are mainly included glycerophospholipid,sphingolipid and arachidonic acid metabolism;Spearman analysis revealed a strong correlation between differential intestinal flora and differential metabolites in hippocampal tissue.Conclusion SGHWT may exert antidepressant effects by adjusting the abundance of various genera in the intestinal flora and regulating the expression of small molecule metabolites in hippocampal tissues.
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