Screening of Differential Genes in Notch Signaling Pathway in Cisplatin-Induced Liver Injury Mice and Its Regulation by Acupuncture and Moxibustion
Objective To investigate the mechanism of acupuncture in improving liver injury by screening the significant differential genes of the Notch signaling pathway in the liver tissue of cisplatin(DDP)-induced mice.Methods Sixty male SPF mice were randomly divided into a blank group,model group,acupuncture group,moxibustion group,blocker acupuncture group,and blocker moxibustion group.The blank group was injected with 0.9%NaCl solution intraperitoneally at a dose of 10 mg·kg-1,and the other groups were injected with the same dose of DDP solution intraperitoneally,and the model was successfully established after 24 h.The acupuncture group and blocker acupuncture group were treated with"Dazhui"(GV 14),"Ganshu"(BL 18),"Shenshu"(BL 23)and"Zusanli"(ST 36),the moxibustion group and the blocker moxibustion group were treated with the same acupoint intervention,and the blocker group was injected with the same dose of DAPT intraperitoneally 30 minutes before treatment.After 5 days of continuous intervention,the left lateral lobe of the liver was taken for DEGseq,immunohistochemistry,and transmission electron microscopy to observe the ultrastructure of the liver on the 6th day.Results DEGseq preliminarily screened 57 differential genes related to the Notch signaling pathway,and 7 hotspot genes Notch1,Hes1,Lfng,Adam17,Numb,Hdac1,and Notch2 were closely related to liver injury.Compared with the blank group,the contents of Notch1,Hes1,Lfng,and Adam17 proteins in the model group were further screened by immunohistochemistry,and the differences were statistically significant(P<0.05).Compared with the model group,the contents of Notch1,Hes1,Lfng,and Adam17 proteins in the remaining four groups were reduced,with statistically significant differences(P<0.05).There was no significant difference between the Numb,Hdac1,and Notch2 groups(P>0.05).Pathological acupuncture showed that acupuncture had an obvious repair effect on the structure of hepatocytes and was superior to the blocker group.Conclusion Acupuncture and moxibustion may inhibit the abnormal activation of the Notch signaling pathway by benign regulation of the expression of differential genes Notch1,Hes1,Lfng,and Adam17 are related to the significance of the Notch signaling pathway,can improve the microstructure of damaged hepatocytes and reduce liver damage caused by DDP chemotherapy,which may be one of the mechanisms for acupuncture to reduce liver damage caused by DDP.
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