Network Pharmacological Analysis of Buyang Huanwu Decoction in Protecting Acute Spinal Cord Injury
Objective To analyze the active components of Buyang Huanwu Decoction and its mechanism of action in protecting acute spinal cord injury.Methods Tradition Chinese Medicine Systems Pharmacology Database and Analysis Plaform,Traditional Chinese Medicine Integrated Database and Traditional Chinese Medicine Integrated Database were used to extract the compound composition and compound target relationship of Buyang Huanwu Decoction.Gene Cards,OMIM and DigSee websites were used to extract the disease targets of acute Spinal cord injury.Vennny map is drawn through online Venn website to obtain intersection targetsand relevant information was imported into CytoscapeCytoscape3.7.2 to obtain the compound-target-disease network.According to the intersection targets,the STRING website obtained the protein interaction network,the R language V4.1.1 carried on the GO and KEGG enrichment analysis.Finally,molecular docking technology and pharmacological experiments were used to verify.Results After preliminary screening,many key traditional Chinese medicine ingredients such as quercetin,luteolin,kaempferol,and isorhamnetin may be involved in regulating nuclear receptor coactivator 2(NCOA2)and heat shock protein 90 ɑ(HSP90AA1),prostaglandin endoperoxide synthetase 2(PTGS2),prostaglandin endoperoxide synthetase 1(PTGS1),Jun proto-oncogene(JUN),protein kinase B ɑ Core targets such as(AKT1)and interleukin-6(IL-6)play a protective role on ASCI.KEGG enrichment analysis involved AGE-RAGE signaling pathway,interleukin-17 signaling pathway,tumor necrosis factor signaling pathway and other complications of diabetes.Meanwhile,animal experiments have also confirmed that the mammalian rapamycin target protein(mTOR)signaling pathway is also the core pathway of Buyang Huanwu Tang in protecting ASCI.Conclusion Buyang Huanwu Tang may protect ASCI through multiple targets,multiple functions,and multiple pathways.PTGS1,PTGS2,AKT,Beclin 1,and mTOR may be the core targets,while mTOR pathway may be the core pathway that plays a protective role.
NETL
NSTL
万方数据
