Identification of Protein Kinases SnRK2 Family in Codonopsis pilosula and its Correlation with Transcription Factors
Objective Through the identification of C.pilosula protein kinase SnRK2 family genes and the preliminary analysis of its correlation with transcription factors,this study provides data support for the analysis of the mechanism of C.pilosula root rot resistance and the breeding of new varieties with high resistance and quality.Methods Based on C.pilosula RNA-seq,using bioinformatics method to systematically study the CpSnRK2 gene family.Results Six CpSnRK2(CpSnRK2.1-CpSnRK2.6)genes were identified and these were devided into three subfamilies.And the motif among the same subfamily members was similar.The expression pattern analysis indicated that the expression levels of CpSnRK2 and transcription factors(WRKY,MYB,ERF and bHLH)had temporal differences were in response to Fusarium oxysporum.At the same time,CpSnRK2 genes were significantly correlated with transcription factors WRKY,MYB,ERF and bHLH.Conclusion This study revealed that CpSnRK2(CpSnRK2.4,CpSnRK2.6)and transcription factors WRKY,MYB,ERF and bHLH jointly regulate the response to Fusarium oxysporum,providing important clues for further research on the response of CpSnRK2 to root rot of C.pilosula.
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