Study on Cold and Heat Properties of Raw Gardeniae Fructus,Stir-Baked Gardeniae Fructus,and Gardeniae Fructus Praeparatus Based on Substance and Energy Metabolism in Normal Rats
Methods The male SD rats were randomly divided into control group,raw Gardeniae Fructus group,stir-baked Gardeniae Fructus group,and Gardeniae Fructus Praeparatus group.The rats of raw Gardeniae Fructus group,stir-baked Gardeniae Fructus group,and Gardeniae Fructus Praeparatus group were administrated with the water extract of raw Gardeniae Fructus,stir-baked Gardeniae Fructus and Gardeniae Fructus Praeparatus,respectively,and the rats of control group were given the normal saline for 9 days.The changes of body weight and anal temperature during administration were record.Enzyme linked immunosorbent assay(ELISA)was used to detect the expression levels of GCK,PFK,PK,Acetyl-CoA,PDH,CS,ICD,α-KGDHC,PYGL,GSK-3,ATGL,CCR,COX,ATPs,ADK,Na+-K+-ATPase,ATP,ADP,NAD+,and NADH.The expression of key proteins in AMPK/mTOR signaling pathway were detected by immunohistochemistry method.Results Compared with the control group,raw Gardeniae Fructus significantly reduced the body weight,anal temperature,the levels of PK,PDH,Acetyl-CoA,CS,ICD,PYGL,GSK-3,ATGL,CCR,ATPs,ADK,Na+-K+-ATPase,ATP,NAD+,NADH,AMPK,significantly elevated the level of mTOR(P<0.01),the levels of GCK,PFK,α-KGDHC,COX,ADP(P<0.05),stir-baked Gardeniae Fructus significantly reduced the body weight,anal temperature,the levels of PK,PDH,Acetyl-CoA,CS,ICD,PYGL,ATGL,ATPs,ADK,Na+-K+-ATPase,ATP,NAD+,NADH,significantly elevated the level of mTOR(P<0.01),the levels of PFK,α-KGDHC,GSK-3,COX,ADP,AMPK(P<0.05),Gardeniae Fructus Praeparatus significantly reduced the anal temperature,the levels of PK,Acetyl-CoA,CS,ICD,PYGL,ATGL,ATPs,ADK,NAD+,significantly elevated the level of mTOR(P<0.01),the body weight,the levels of PDH,Na+-K+-ATPase,ATP,NADH(P<0.05).Compared with raw Gardeniae Fructus group,stir-baked Gardeniae Fructus significantly increased the levels of PK,PDH,Acetyl-CoA,ICD,ADK,NADH(P<0.01),the level of PDH(P<0.05).Gardeniae Fructus Praeparatus significantly increased the levels of PK,PDH,Acetyl-CoA,CS,ICD,ADK,Na+-K+-ATPase,NAD+,NADH(P<0.01),the levels of GSK-3,ATPs,AMPK(P<0.05).Compared with stir-baked Gardeniae Fructus group,Gardeniae Fructus Praeparatus significantly increased the levels of Na+-K+-ATPase,NADH(P<0.01).Conclusion The raw Gardeniae Fructus,stir-baked Gardeniae Fructus,and Gardeniae Fructus Praeparatus could significantly reduce the levels of substance and energy metabolism,indicating that the three processed products of Gardenia Fructus all showed the cold property,which was consistent with the record in Chinese Pharmacopoeia.The levels of substance and energy metabolism of rats in the Gardeniae Fructus Praeparatus group were significantly higher than those of rats in the stir-baked Gardeniae Fructus group,while the levels of substance and energy metabolism of rats in the stir-baked Gardeniae Fructus group were significantly higher than those of rats in the raw Gardeniae Fructus,suggesting that the cold property of the Gardeniae Fructus Praeparatus was weaker than that of the stir-baked Gardeniae Fructus and the cold property of the stir-baked Gardeniae Fructus was weaker than that of raw Gardeniae Fructus.Obiective To clarify the scientific connotation of the changes in cold and heat properties of raw Gardeniae Fructus,stir-baked Gardeniae Fructus,and Gardeniae Fructus Praeparatus based on substance and energy metabolism after drug intervention in the normal rats.
Raw Gardenia FructusStir-baked Gardenia FructusGardenia Fructus PraeparatusCold and heat propertiesSubstance metabolismEnergy metabolism
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