基于白术内酯及总多糖结合外观性状评价白术饮片等级质量
Evaluation of Atractylodis macrocephalae Rhizoma Decoction Pieces Quality Grade Based on Appearance Characteristics and Atractylodes Lactone and Total Polysaccharide
杜洪志 1郑英 2陈志琳 1张敏杰 1李玮1
作者信息
- 1. 贵州中医药大学药学院 贵阳 550025
- 2. 绥阳县中医医院 绥阳 563300
- 折叠
摘要
目的 通过对不同等级白术饮片进行研究,完善白术质量评价方法,为传统白术饮片等级划分提供理论依据.方法 根据传统分级依据,将白术饮片分为3个等级.采用HPLC及UV法,测定36批白术饮片不同等级中白术内酯Ⅰ、Ⅱ、Ⅲ及总多糖的含量,结合2020版《中国药典》,将白术不同等级饮片的外观性状与内在质控指标进行相关性分析.结果 根据外观性状及多个质控指标的含量测定,通过主成分分析、聚类分析等,白术一级饮片质量明显优于二级、三级饮片(P<0.05),且二级、三级饮片之间无显著性差异(P>0.05),表明白术饮片可分为2个等级,不同等级饮片与白术内酯及总多糖含量高低呈正相关.结论 基于多个质控指标结合外观性状的白术饮片等级划分,可为该饮片质量控制与等级研究提供一定科学依据.
Abstract
Objective Through the study of different grades for Atractylodis macrocephalae Rhizoma decoction pieces,improve the quality evaluation method,and provide theoretical basis for the classification of Atractylodis macrocephalae Rhizoma.Methods According to the traditional classification,the Atractylodis macrocephalae Rhizoma decoction pieces was divided into three grades.By HPLC and UV method,the determination of atractylodes lactone Ⅰ,Ⅱ,Ⅲ and the content of total polysaccharide for 36 batches of different grades of Atractylodis macrocephalae Rhizoma decoction pieces.According to the 2020 edition of Chinese pharmacopoeia,the correlation between the appearance traits of different grades of Atractylodis macrocephalae Rhizoma decoction pieces and the internal quality control indexes was analyzed.Results According to the appearance characters,multiple quality control index of content determination,through the principal component analysis,cluster analysis and so forth.The quality of first-grade decoction pieces of Atractylodis macrocephalae Rhizoma is significantly better than the second-grade and third-grade decoction pieces(P<0.05),and there is no significant difference between second-grade and third-grade decoction pieces(P>0.05).As a result,the Atractylodis macrocephalae Rhizoma decoction pieces can be divided into two grades,different grades with the content of atractylodes lactone and total polysaccharide were positively correlated.Conclusion The classification of Atractylodis macrocephalae Rhizoma decoction pieces based on multiple quality control indexes combined with appearance characters can provide a scientific basis for the quality control and grade study of this decoction piece.
关键词
白术饮片/白术内酯/总多糖/外观性状/等级研究Key words
Atractylodis macrocephalae Rhizoma decoction pieces/Atractylodes lactone/Total polysaccharide/Appearance characteristics/Grade study引用本文复制引用
基金项目
贵州省科学技术厅重大专项课题(黔科合重大专项字[2015]6009-4号)
国家中医药管理局2015年中医药部门公共卫生专项(201586号)
贵州省中医药管理局2019年度科学技术研究重点项目(QZYYZD-2019-01)
出版年
2024
NETL
NSTL
万方数据