目的 建立一测多评法同时测定凤尾草配方颗粒中4个成分，并优化其总黄酮提取工艺。方法 采用(超高效液相色谱)UPLC法，以野漆树苷为内标物，建立一测多评(Quantitative analysis of multi-compounds by single-marker，QAMS)法计算凤尾草配方颗粒中忍冬苷、木犀草素、芹菜素的相对校正因子，考察其耐用性。采用外标法测定凤尾草配方颗粒中4个成分含量，比较一测多评法计算值与外标法实测值的差异。通过单因素实验研究超声时间、乙醇体积分数、料液比、超声功率对凤尾草配方颗粒总黄酮提取率的影响。在此基础上，进行3因素3水平的Box-Behnken实验，优化总黄酮的提取工艺，并进行验证实验。结果 7批凤尾草配方颗粒中野漆树苷的含量范围为0。035%-0。056%，忍冬苷、木犀草素、芹菜素的QAMS法含量测定结果范围分别为0。085%-0。167%、0。014%-0。028%、0。004%-0。008%，均与外标法测定结果无显著差异。总黄酮的最佳提取条件为以80%乙醇水、料液比1:20、超声提取40 min，平均提取率为24。46 mg·g-1。结论 建立的一测多评法准确可行，基于Box-Behnken响应面法优选的总黄酮提取工艺简单可行，可为凤尾草配方颗粒的质量评价提供参考。
Determination of Four Compounds and Optimization of Extraction Technology of Total Flavonoids in Pteris multifida Formula Granules
Objective To establish a quantitative analysis of multi-compounds by single-marker(QAMS)method for the simultaneous determination of four compounds in formula granules of Pteris multifida and optimize the extraction process of total flavonoids.Methods The relative correction factors(RCFs)of lonicerin,luteolin and apigenin in the formula granules of Pteris multifida were calculated by using the UPLC method with rhoifolin as the internal reference,and their durability was investigated.The external standard method(ESM)was used to determine the content of four compounds in the formula granules of Pteris multifida,and the difference between the calculated values and the measured values was compared.The effects of ultrasonic time,ethanol volume fraction,solid-liquid ratio and ultrasonic power on the extraction rate of total flavonoids were studied by single factor experiment.On this basis,Box-Behnken test with three factors and three levels was conducted to optimize the extraction process of total flavonoids,and the verification experiment was conducted.Results The content of rhoifolin in the formula granules was 0.035%-0.056%,and the content of lonicerin,luteolin and apigenin by QAMS method was 0.085%-0.167%,0.014%-0.028%and 0.004%-0.008%,respectively,which had no significant difference with the external standard method.The optimal extraction conditions of total flavonoids were 80%ethanol water,solid-liquid ratio 1:20,40 minutes extraction,the average extraction rate was 24.46 mg·g-1.Conclusion The established QAMS method was accurate and feasible,and the optimized extraction process of total flavonoids based on Box-Behnken response surface method was simple and feasible,which could lay a foundation for the quality evaluation of the Pteris multifida formula granules.
Pteris multifida formula granulesQuantitative analysis of multi-compounds by single-markerTotal extraction rate of total flavonoidsExtraction technologyBox-Behnken test
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