Identification and Expression Analysis of WRKY Gene Family in Chrysanthemum nankingense
Objective Bioinformatics methods were used to identify and analyze members of the WRKY gene family in Chrysanthemum nankingense,which provided a theoretical basis for further studies on the regulation of WRKY transcription factors on the biosynthesis of terpenes in C.nankingense.Methods Online tools such as HMMER,BLAST,Pfam and NCBI were used to screen and identify the WRKY gene family members in the brain genome of C.nankingense.ExPASy,WOLF PSORT and other online tools for physical and chemical properties analysis;ClustalW,IQ-Tree,MEME and other software for system evolution,conservative domain analysis;TBtools software for chromosome localization,tandem replication,gene expression patterns and visual analysis.Results A total of 110 CnaWRKYs were identified,whose ORF length was 246-2367 bp,amino acid number was 81-788 AA,molecular weight was 9463.98-87062.38 Da,theoretical isoelectric point was 4.63-10.15,and 93 Cnawrkys were predicted to be located in the nucleus.CnaWRKYs protein was divided into 3 groups by phylogenetic analysis,and group Ⅱ was divided into 5 subgroups.Protein domains are abundant,and the same group of CnaWRKYs protein domains are highly conserved with similar conserved motifs.There were 109 WRKY genes unevenly distributed on 9 chromosomes,and one WRKY gene was distributed on Scalfold25.The gene expression heat map showed that 108 CnaWRKYs genes were expressed at the whole level,and the expression abundance of WRKY gene family members in different tissues was significantly different.Conclusion The WRKY gene family members of C.nankingense were systematically analyzed to provide theoretical basis for further analysis of the role of CnaWRKYs gene in the biosynthesis of volatile aromatic compounds terpenes in C.nankingense and its regulatory mechanism.
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