The Regulatory Role of Electroacupuncture on Caspase-1 and its Downstream IL-1β and IL-18 in Cornea of Type 2 Diabetic Dry Eye Rats
Objective Dry eye is a common complication of diabetes.Electroacupuncture is clinically effective in the treatment of dry eye in diabetes,but its mechanism is still unclear.The aim of this study is to reveal how electroacupuncture regulates Caspase-1 and its downstream IL-1 β and 18,to improve keratitis in type 2 diabetic dry eye rats,thereby alleviating the potential mechanism of ocular surface signs in type 2 diabetic dry eye rats.Methods The model of type 2 diabetic rats with dry eye was established by intraperitoneal injection of 1％streptozotocin,and blank group,model group,electroacupuncture group,sham acupuncture group and fluminolone group were established.Selecting Jingming,Zanzhu,Sizhukong,Taiyang,and Tongziliao for electroacupuncture intervention.Random blood glucose levels,tear film rupture time,corneal fluorescein sodium staining,phenol red cotton thread test,corneal mechanical perception threshold,and blink frequency were measured before modeling,after modeling,and after treatment to observe the overall and ocular surface conditions of the rats.HE staining method was used to observe the morphology of rat cornea.Immunohistochemical,Western blot,and RT-PCR methods were used to determine the expression level of Caspase-1,IL-1 β and IL-18 in corneal tissue.Results Electroacupuncture can improve tear film rupture time,tear secretion,corneal sensitivity,promote corneal epithelial repair,and inhibit the expression level of Caspase-1,and its downstream IL-1 β and IL-18 in corneal tissue of type 2 diabetic dry eye rats.Conclusion The therapeutic effect of electroacupuncture on the ocular surface of type 2 diabetic dry eye rats is related to the anti-inflammatory effect of inhibiting the expression of Caspase-1 and its downstream IL-1 β and IL-18 in cornea.
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