Objective Endophytic fungi were isolated and purified from fresh Glycyrrhiza glabra L.and strains capable of producing secondary metabolites with inhibitory effects on adenosine deaminase,β-lactamase,xanthine oxidase,and tyrosinase were screened.Methods Four types of culture media were used to isolate and purify endophytic fungi using tissue homogenization method.Subsequently,the secondary metabolites of endophytic fungi were evaluated using an activity assessment platform.Results A total of 131 strains of endophytic fungi were isolated from the plant,among which 34 strains exhibited distinct morphologies and special characteristics.The mycelial extract of hypha G(1-5)(s)showed good adenosine deaminase inhibitory activity(inhibition rate:98.58％)and β-lactamase inhibitory activity(inhibition rate:93.15％).and β-lactamase(inhibition rate:93.15％).Strain G6 exhibited excellent xanthine oxidase inhibitory activity(inhibition rate:91.38％)in the ethyl acetate phase.These strains were identified as Penicillium sp.and Aspergillus sp.Conclusion Glycyrrhiza glabra L.harbors a diverse range of endophytic fungi with promising research potential.Further investigation is warranted to provide important technical data for the protection and in-depth applications of Glycyrrhiza glabra L..
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