Effects of Bursaphelenchus xylophilus infestation on physiological and biochemical responses of Pinus tabuliformis
To study the changes of some physiological and biochemical substances in the needles of Pinus tabuliformis after infestation with Bursaphelenchus xylophilus,the 5-year-old potted seedlings of P.tabuliformis were selected as the research objects.The activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),phenylalanine ammonialyase(PAL),contents of osmotic regulatory substances and secondary metabolites in P.tabuliformis needles were tested.The results showed that:the POD activity of P.tabuliformis needles was lower than that of the control group five days and 20 days after inoculation.The CAT activity was significantly higher than the control group 30 days after inoculation.SOD activity decreased slightly at first and then increased gradually,peaking at 30 days after inoculation and significantly higher than the control group.The PAL activity showed a trend of continuous increase,and reached a significant difference level compared with the control group 20 and 30 days after inoculation.The content of soluble protein initially increased and then decreased,peaking at 10 days after inoculation and was significantly higher than the control group at both 10 and 20 days after inoculation.The betaine content in the experimental group reached the peak value five days after inoculation,which was significantly higher than that in the control group.The flavonoid content increased initially and then decreased,being significantly higher than that in the control group 20 and 30 days after inoculation.Therefore,the activity of various defense enzymes and physiological and biochemical substances in P.tabuliformis seedlings would change dynamically when infected by B.xylophilus,and induce P.tabuliformis to produce defense response.
Bursaphelenchus xylophilusPinus tabuliformisantioxidant enzymephysiology and biochemistrysecondary metabolite
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