Response mechanism of Juglans sigillata and Juglans regia×Juglans sigillata'Yunxin Yunlin'to Colletotrichum gloeosporioides
To evaluate the difference in resistance of Juglans sigillata and Juglans regia×Juglans sigillata'Yunxin Yunlin'to anthracnose,the incidences of anthracnose on the peels were observed by field investigations and inoculation assay indoor.The peels of two cultivars of walnut were used as the test materials,the differences of metabolites in peels between the two cultivars of walnut after inoculation with Colletotrichum gloeosporioides were determined by extensive targeted metabolomics.At the same time,KEGG analysis was carried out,and the changes of defense enzyme activities in the peels of two cultivars of walnuts before and after inoculation with C.gloeosporioides were determined.The result showed that J.sigillata had stronger resistance to anthracnose than J.regia×J.sigillata'Yunxin Yunlin';the number of phenolic compounds up-regulated in the peel of J.sigillata after inoculating were more than that in the peel of J.regia×J.sigillata'Yunxin Yunlin'after infection,and the phenylpropanoid metabolic pathway was the main pathway of J.sigillata against anthracnose;2',4',6',3,4-pentahydroxychalcone,quercetin and 3,7-O-dimethylquercetin showed significant up-regulation.The PPO and POD enzyme activities of J.sigillata increased after inoculation,and were significantly higher than those of J.regia×J.sigillata'Yunxin Yunlin'from 72 to 120 hours after inoculation.The resistance of J.sigillata to anthracnose was produced through the metabolism of phenolic compounds.
万方数据
维普
