To identify the pathogen of rust disease on Salix matsudana,the morphological characteristics of urediospores were observed using both light microscope and scanning electron microscope.The genomic DNA of urediospores were extracted,PCR amplification was performed on the ITS and LSU of ribosomes to further construct a phylogenetic tree.The pathogen was found to cluster with Melampsora ferrinii on the phylogenetic tree.Combined with morphological identification,the pathogen was determined to be M.ferrinii Toome&Aime.M.ferrinii Toome&Aime was a new record species in the Northeast region of China,and S.matsudana was reported as a new host plant for this fungus internationally for the first time.
关键词
锈病/旱柳/铁锈栅锈菌/系统发育分析/新记录
Key words
rust disease/Salix matsudana/Melampsora ferrinii/phylogenetic analysis/new record
维普
万方数据