Isolation and Identification of Yeast YH1 and Its Growth Characteristics
In order to obtain a strain of Saccharomyces cerevisiae and obtain the growth characteristics of the strain under certain conditions,a strain YH1 was isolated and purified from the soil samples under the grape trellis,and the strain was identified by morphology and molecular biology,and its genetic stability was evaluated.The results showed that the strain was Saccharomyces cerevisiae,and the biomass of the strain was maintained 5.43-5.92 g·500 mL-1,and it had good genetic stability.The growth characteristics of this strain were measured by measuring the change curves of amino acid nitrogen,pH,the total sugars and lethal temperature of the strain in yeast leaching peptone glucose(YPD)agar medium at different time.The results showed that the sugar consumption of the strain was mainly in the first 6 h,the consumption of nitrogen was higher than that of nitrogen-containing compounds,and the pH showed a downward trend and then an upward trend,which remained at 5.5-6.5 as a whole,and the number of strains tended to grow flat after 18 h.The strain was heated for 10 min at the temperature higher than 58℃with little growth or no growth,the optimal fermentation conditions of the strain at 27℃and 180 r·min-1 were obtained.
yeastSaccharomyces cerevisiaeisolation and identificationevaluation of genetic stabilitygrowth characteristics
NETL
NSTL
万方数据
维普
