Effects of Tea Polyphenols on Intestinal Barrier Function in Heat Stressed Broilers
In order to explore the effects of tea polyphenols on the intestinal barrier function of heat stressed broilers,500 healthy 20 day old white feather broiler chickens weighing(800.00±2.00)g were selected as the research subjects.They were randomly divided into five groups using a random number table method,with 100 chickens in each group.Each group had 10 replicates,with 10 replicates each.Each replicates had half of the male and female chickens,and were divided into group A[a blank control group(room temperature+basic diet)],group B[heat stress treatment(heat stress+basic diet)],group C[the low dose group of tea polyphenols(heat stress+basal diet+0.05%tea polyphenols)],group D[the medium dose group of tea polyphenols(heat stress+basal diet+0.10%tea polyphenols)],and group E[the high dose group of tea polyphenols(heat stress+basal diet+0.15%tea polyphenols)];the test period of this experiment was 21 days.On the 21st day of the experiment,the transmembrane resistance value of intestinal epithelial cells and the permeability of intestinal epithelium to fluorescein isothiocyanate glucan in white feather broiler chickens were detected using the Yusi perfusion technique.The results showed that compared with the group A,the transmembrane resistance of the jejunum in the group B of white feather broilers was significantly reduced,and the difference was significant;the permeability of fluorescein isothiocyanate glucan in the jejunum of white feather broiler chickens treated with heat stress was significantly increased,and the difference was significant(P<0.05);the groups C and D of tea polyphenols significantly reduced the transmembrane resistance of the jejunum in white feather broiler chickens,and the difference was significant(P<0.05);the permeability of fluorescein isothiocyanate glucan in the jejunum of white feather broiler chickens was significantly increased in the groups C and D of tea polyphenols,and the difference was significant(P<0.05).Compared with the group B,the groups C,D and E of tea polyphenols significantly increased the jejunal transmembrane resistance of white feather broilers,and the difference was significant(P<0.05);the per-meability of fluorescein isothiocyanate glucan in the jejunum of white feather broiler chickens was significantly reduced in the groups C,D and E of tea polyphenols,and the difference was significant(P<0.05).The above results indicated that heat stress could cause damage to the intestinal mucosal barrier and increase the permeability of the barrier in white feather broilers,while tea polyphenols could play a repairing role in the intestinal barrier damage caused by heat stress in white feather broilers.
tea polyphenolsheat stressmeat chickenintestinal barrier function
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万方数据
维普
