Isolation and Identification of a Strain of Lactobacillus salivarius from Goose
In order to select lactic acid bacteria strains which are specific to geese,a strain of Lactobacillus derived from the feces of healthy geese was identified by physiological,biochemical and 16S rRNA.The experiments were carried out for the acid production curve,bile salt tolerance,survival rate in artificial gastric and intestinal fluids,antibacterial activity,and antibiotic resistance.The identification result of the strain named NKXM002 was a Lactobacillus salivus,which had a strong acid-producing and a pH of 3.7.The survival rates of the strain were 78.33%and 80.84%after 3 h culture in artificial gastric fluid and artificial intestinal fluid.The strain could be survive in the Man Rogosa Sharpe(MRS)medium containing 0.5%bile salt.The strain could inhibit the growth of Escherichia coli,Salmonella and Staphylococcus aureus.The strain was antiboiotic resistant to amikacin,ampicillin,ampicillin/sulbactam,metronidazole,megamycin and vancomycin.In conclusion,NKXM002 had good probiotic properties and could be used as a candidate strain for probiotics.
gooseLactobacillus salivariusisolation and identificationantibacterial activityantibiotic resistance
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