Experimental Study on Anaerobic Fermentation of Selenium Rich Mulberry Leaf Silage Feed
In order to explore the microbial changes in the anaerobic fermentation process of mulberry leaf silage and the reasons for the poor effect of mulberry leaf direct silage,the selenium rich mulberry leaf silage was prepared by using probiotic compound starter as the modifier,corn flour as the substrate,and the anaerobic fermentation contrast test of selenium rich mulberry leaf silage was carried out in four groups of A,B,C,D.Group A was mulberry leaf fermentation(control group),group B was mulberry leaf+probiotic compound starter mixed fermentation,group C was mulberry leaf+corn meal mixed fermentation,group D was mulberry leaf+probiotic compound starter+corn meal mixed fermentation.The results showed that the quality of mulberry leaves silage alone was poor,due to the low soluble sugar content in mulberry leaves,which could not produce sufficient lactic acid to inhibit the fermentation of spoilage bacteria,affecting the quality of silage;the addition of lactic acid bacteria group improved the abundance advantage of lactic acid bacteria in fermentation to quickly inhibit miscellaneous bacteria in silage;the addition of corn flour increased the soluble sugar,and sufficient soluble sugar made the epiphytic lactic acid bacteria of mulberry leaves produce sufficient lactic acid,effectively inhibited the growth of putrefactive bacteria,promoted the growth of lactic acid bacteria,and increased their acid production;mulberry leaf+probiotic compound starter+corn flour were mixed and fermented at 25℃,pH 6.5,material moisture content 65%,and fermentation time 60 days.After fermentation,selenium rich mulberry leaf silage increased its nutritional components such as protein and fat content,and produced rich bioactive substances such as lactic acid and acetic acid,which improved the nutritional value and bioavailability of the feed.After fermentation,the content of crude fiber and anti nutritional factors in mulberry leaves significantly decreased,greatly improving and enhancing their feeding value.
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