With Forsythia suspensa fruit as raw material,using ultrasonic auxiliary water alcohol extract Forsythia suspensa crude polysaccharides,the yield of polysaccharides was used as the investigation index,based on the results of the orthogonal test combined with orthogonal test of Forsythia suspensa crude polysaccharides preparation process optimization,and using DPPH method and ABTS method to investigate its in vitro antioxidant activity,bacteriostatic method to investigate the antibacterial activity of Forsythia suspensa crude polysaccharides.The results showed that the best extraction process of Forsythia suspensa crude polysaccharides follows:the solid-liquid ratio was 1:20(g:mL),the extraction temperature was 70℃,the ultrasonic power was 70 W,and the extraction time was 50 min.The Forsythia suspensa crude polysaccharides extraction rate was 1.98%.The results showed that Forsythia suspensa crude polysaccharides showed good growth inhibition on S.aureus and Escherichia coli,and better inhibition on S.aureus than E.coli.At 2.5 mg/mL,the mean diameter of E.coli and 11.73 mm of S.aureus.Forsythia suspensa polysaccharide cleared DPPH·and ABTS·and showed a good mass concentration-effect relationship.Forsythia suspensa polysaccharides not only has certain antibacterial activity against food spoilage bacteria,but also has good in vitro antioxidant activity has the potential of developing into food preservation preservatives.
Forsythia suspensa polysaccharidehot water extraction methodantioxidant activityantibacterial activity
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