首页|丹参LBD基因家族的特性鉴定与抗热性分析

丹参LBD基因家族的特性鉴定与抗热性分析

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利用生物信息学方法,对丹参LBD(later organ boundaries domain)家族成员(SmLBDs)进行了系统的筛选和鉴定,并分析了其在高温胁迫(37℃)下的表达模式.结果表明,丹参中共有 52 个SmLBDs,可分为groupⅠ、groupⅡ、groupⅢ、groupⅣ、groupⅤ、groupⅥ和groupⅦ 共 7 组.52 个SmLBDs 编码蛋白的氨基酸残基数为 103~298,相对分子质量为11.29~31.72 kDa,等电点分布于 4.44~10.71,均为亲水性蛋白,且大多定位在细胞核中.此外,分析 52 个SmLBDs在高温胁迫(37℃)下的表达模式发现,7 个基因的表达水平下降,37 个基因的表达水平上升,尤其是SmLBD8、SmLBD40 和SmLBD44 的表达水平显著提高,表明这些基因可能与丹参的抗热性相关.
Genome-wide Identification and Analysis of LBD Gene Family in Salvia miltiorrhiza
The members of the later organ boundaries domain(LBD)family of Salvia miltiorrhia LBD(SmLBDs)were systematically screened and identified using bioinformatics methods,and their expression patterns were analyzed under high temperature stress(37 ℃).The results showed that there were 52 SmLBDs in Salvia miltiorrhiza,which could be divided into 7 groups:groupⅠ,groupⅡ,groupⅢ,groupⅣ,groupⅤ,groupⅥ and groupⅦ.The amino acid residues of the 52 proteins encoded by SmLBDs ranged from 103 to 298,the relative molecular weights ranged from 11.29 to 31.72 kDa,and the isoelectric points were distributed from 4.44 to 10.71.All of 52 SmLBDs were hydrophilic proteins,and most of them were located in the nucleus.In addition,the expression patterns of 52 SmLBDs under high temperature stress(37 ℃)were analyzed,and the expression levels of 7 genes were decreased,and 37 genes were increased,especially the expression level of SmLBD8,SmLBD40 and SmLBD44 was significantly increased,indicating that these genes may be related to the heat resistance of Salvia miltiorrhiza.

Salvia miltiorrhizaLBD familysubcellular localizationhigh temperature stress

李万、樊昕华

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商洛学院生物医药与食品工程学院,陕西商洛 726000

丹参 LBD家族 亚细胞定位 高温胁迫

陕西省教育厅科研项目

23JK0415

2024

商洛学院学报
商洛学院

商洛学院学报

影响因子:0.412
ISSN:1674-0033
年,卷(期):2024.38(2)
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