超高效液相串联三重四极杆质谱法测定果冻中胭脂红酸
Determination of Carminic Acid in Jelly by Ultra High Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry
汪强1
作者信息
- 1. 马鞍山市食品药品检验和药品不良反应监测中心,安徽马鞍山 243000
- 折叠
摘要
建立了超高效液相色谱串联三重四极杆质谱仪测定果冻中胭脂红酸含量的方法.样品通过盐酸水溶液酸化提取,并经过HLB固相萃取柱净化后,上机测定,基质外标法定量.胭脂红酸在5～200 ng·mL-1线性关系良好,相关系数＞0.999,方法的检出限为3.36 ng·g-1,定量限为11.21 ng·g-1.在不同浓度的加标水平下,回收率为 91.2%～98.2%,相对标准偏差均＜2%.本方法污染小,操作简便,结果准确,适用于果冻中胭脂红酸的检测.
Abstract
A method for the determination of carminic acid content in jelly using ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometer was established.The sample was acidified and extracted with hydrochloric acid aqueous solution,and after purification with an HLB solid-phase extraction column,it was measured on an instrument and quantified by the matrix external standard method.The linearity of carminic acid was good in the range of 5～200 ng·mL-1 with the correlation coefficient＞0.999.The limit of detection of the method was 3.36 ng·g-1,and the limit of quantification was 11.21 ng·g-1.The recoveries ranged from 91.2%to 98.2%at the spiked levels of different concentrations,and the relative standard deviations were all＜2%.The method is characterized by low contamination,simple operation,accurate results and suitable for the determination of carminic acid in jelly.
关键词
胭脂红酸/果冻/超高效液相色谱串联三重四极杆质谱法Key words
carminic acid/jelly/ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据