Determination of Carminic Acid in Jelly by Ultra High Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry
A method for the determination of carminic acid content in jelly using ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometer was established.The sample was acidified and extracted with hydrochloric acid aqueous solution,and after purification with an HLB solid-phase extraction column,it was measured on an instrument and quantified by the matrix external standard method.The linearity of carminic acid was good in the range of 5～200 ng·mL-1 with the correlation coefficient＞0.999.The limit of detection of the method was 3.36 ng·g-1,and the limit of quantification was 11.21 ng·g-1.The recoveries ranged from 91.2%to 98.2%at the spiked levels of different concentrations,and the relative standard deviations were all＜2%.The method is characterized by low contamination,simple operation,accurate results and suitable for the determination of carminic acid in jelly.
carminic acidjellyultra performance liquid chromatography-triple quadrupole tandem mass spectrometry
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NSTL
万方数据
维普
