Improvement of the Determination Method for Total Flavonoids in Propolis Soft Capsules
Propolis is rich in a large amount of total flavonoids,which have rich pharmacological effects.When testing propolis soft capsules with reference to GB/T 20574-2006,the solution became turbid after adding a colorimetric agent to a constant volume of water,which affected the stability of the spectrophotometer absorbance measurement.The pre-treatment conditions,constant volume solution,and colorimetric time were improved,and the method standard curve,detection limit,repeatability,and spiked recovery rate were verified.The results showed that at 415 nm,the content of rutin was 0.2～1.2 mg,and the linear regression equation was y= 0.734 1x-0.005 6(R= 0.999 8),the relative standard deviation was 0.9%.The recoveries were 92.0%～97.0%.
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万方数据
维普
