蜂胶软胶囊中总黄酮测定方法的改进
Improvement of the Determination Method for Total Flavonoids in Propolis Soft Capsules
周璐 1黄婷 1董耀1
作者信息
- 1. 江苏省生产力促进中心,江苏南京 210001;江苏省理化测试中心,江苏南京 210001
- 折叠
摘要
蜂胶中富含大量总黄酮类化合物,黄酮类化合物具有丰富的药理作用.对蜂胶软胶囊参照GB/T 20574-2006检测时,加入显色剂用水定容后,溶液变浑浊,影响分光光度计吸光度测定的稳定性,本文对其前处理条件、定容溶液、显色时间进行优化,并对方法标准曲线、重复性、加标回收率进行验证.结果表明,在 415 nm处,以芦丁为标准品,在含量为0.2～1.2 mg时呈现良好的线性关系,线性回归方程为y=0.734 1x-0.005 6(R=0.999 8),相对标准偏差为0.9%,加标回收率为92.0%～97.0%.
Abstract
Propolis is rich in a large amount of total flavonoids,which have rich pharmacological effects.When testing propolis soft capsules with reference to GB/T 20574-2006,the solution became turbid after adding a colorimetric agent to a constant volume of water,which affected the stability of the spectrophotometer absorbance measurement.The pre-treatment conditions,constant volume solution,and colorimetric time were improved,and the method standard curve,detection limit,repeatability,and spiked recovery rate were verified.The results showed that at 415 nm,the content of rutin was 0.2～1.2 mg,and the linear regression equation was y= 0.734 1x-0.005 6(R= 0.999 8),the relative standard deviation was 0.9%.The recoveries were 92.0%～97.0%.
关键词
蜂胶软胶囊/总黄酮/分光光度法Key words
propolis soft capsules/total flavonoids/spectrophotometry引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据