Optimization of High Performance Liquid Chromatography Method for Determining Vitamin C Content in Different Edible Parts of Laiwu Jitui Onion
In this paper,high performance liquid chromatography(HPLC)was used to determine the vitamin C(L-ascorbic acid)content in the onion stems and leaves of Laiwu Jitui onions,and the method was optimized.The samples were extracted twice with 1.0%oxalic acid solution as the extracting solution by ultrasonic extraction,separated on a Kromasil C18 column,and detected by an ultraviolet detector at 254 nm.The samples were qualitatively analyzed by the retention time,and quantitatively analyzed by the external standard method.The results showed that there was a good linear relationship(R=0.999 5)when the mass concentration of vitamin C was between 2.0 mg·L-1 and 100.0 mg·L-1.The relative standard deviation of the precision test was 1.33%(n=6),the recovery rate was 94.9%～96.1%,and the detection limit of the method was 2.0×10-3 g·kg-1.This testing method is simple and fast,and can accurately determine the vitamin C content in the stems and leaves of Laiwu Jitui onion.
high performance liquid chromatographyLaiwu Jitui oniononion stemonion leavevitamin C
NETL
NSTL
万方数据
维普
