Determination of Dehydroacetic Acid Content in Grain-Related Food by Gas-Chromatography
Objective:To determine the content of dehydroacetic acid in processed food by gas chromatography.Method:The pretreatment conditions and chromatographic conditions were optimized,such as the method of protein precipitation,oil removal,extraction method and time,and the linear relationship,precision,recovery rate and detection limit of the method were investigated.Result:In the range of 1.0～100.0 μg·mL-1,the linear relationship is good,with a correlation coefficient R2>0.999,and the detection limit of the method was 0.000 8 g·kg-1.The sample spiked recovery rate of this method is 85.9%～105.2%and the relative standard deviation was 3.55%～7.64%,which met the requirements of the technical parameters of the method in GB 5009.295-2023.Conclusion:The method have been sensitive,accurate,and have had a good reproducibility,which can be used for the determination of dehydroacetic acid content in grain-related food.
gas chromatographygrain-related fooddehydroacetic acidoptimization of chromatographic conditions
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