雁鹅菌菌丝培养条件优化研究
Study on Condition Optimization of Mycelium Culture of Lactarius deliciosus
刘会敬 1阳焱红 2朱斐淇 2曾静 2莫寒 2杨期辉3
作者信息
- 1. 衡阳师范学院南岳学院 生命科学系,湖南衡阳 421000;衡阳师范学院 生命科学学院,湖南衡阳 421000;南岳山区生物资源保护与利用湖南省重点实验室,湖南衡阳 421000
- 2. 衡阳师范学院南岳学院 生命科学系,湖南衡阳 421000
- 3. 衡阳师范学院 生命科学学院,湖南衡阳 421000
- 折叠
摘要
为优化雁鹅菌菌丝的培养条件,以菌丝生长速度为评价指标,通过单因素试验分别确定雁鹅菌菌丝生长培养基的最佳碳源、氮源以及无机盐,并通过比较不同的营养元素浓度对雁鹅菌菌丝生长速度的影响,确定各营养元素的最佳添加区间,后续采用正交试验法优化雁鹅菌菌丝生长培养基中各营养元素的组合.结果表明,最佳碳源为可溶性淀粉,最佳氮源为胰蛋白胨,最佳无机盐为氯化钙.雁鹅菌菌丝生长培养基的最优组合为可溶性淀粉浓度 5 g·L-1,胰蛋白胨浓度3 g·L-1,培养基中氯化钙浓度1.00 g·L-1.
Abstract
In order to optimize the cultivation conditions of the mycelium of Lactarius deliciosus,the mycelial growth rate was used as an evaluation index.The optimal carbon source,nitrogen source,and inorganic salt for the mycelial growth medium of Lactarius deliciosus were determined through single factor experiments.By comparing the effects of different nutrient element concentrations on the mycelial growth rate of Lactarius deliciosus,the optimal range for adding each nutrient element was determined.Subsequently,the orthogonal experimental method was used to optimize the combination of various nutrient elements in the mycelial growth medium of Lactarius deliciosus.The results indicate that the optimal carbon source is soluble starch,the optimal nitrogen source is tryptone,and the optimal inorganic salt is calcium chloride.The optimal combination of mycelial growth medium for wild goose fungus is soluble starch concentration of 5 g·L-1,tryptone concentration of 3 g·L-1,and calcium chloride concentration of 1.00 g·L-1 in the medium.
关键词
雁鹅菌/菌丝生长/培养条件/正交试验Key words
Lactarius deliciosus/mycelial culture/culture condition/orthogonal experiment引用本文复制引用
基金项目
国家大学生创新创业训练计划(2021)(202112659001)
出版年
2024
NETL
NSTL
万方数据