水产品中组胺快速检测的方法研究
Research on Rapid Detection of Histamine in Aquatic Products
汪开银 1熊丽莎 2陈斌 1黄丹萍 1聂小林 1熊晓辉3
作者信息
- 1. 国家轻工业食品质量监督检测南京站,江苏南京 211800
- 2. 江苏省食品生产安全协会,江苏南京 211816
- 3. 南京工业大学,江苏南京 211800
- 折叠
摘要
目的:建立水产品中组胺快速检测的方法,并进行方法学验证.方法:本研究以偶氮试剂法-比色卡法作为水产品中组胺的快速检测手段.样品中的组胺经提取后,通过正戊醇萃取净化,再与偶氮试剂反应生成橙色化合物,其溶液颜色的深浅在一定范围内与样品中组胺的含量呈正相关,通过组胺标准色阶卡进行目视比色,对样品中组胺进行定性判定.结果:以鲈鱼、秋刀鱼、罗氏虾作为空白基质的检出限为200 mg·kg-1,灵敏度和特异性为100%、假阴性率和假阳性率为0%.结论:该方法快速准确,灵敏度高,适用于水产品中组胺的快速检测.
Abstract
Objective:To establish a rapid method for detecting histamine in aquatic products and validate it through methodological validation.Method:This study used the azo reagent colorimetric card method as a rapid detection method for histamine in aquatic products.After extraction,histamine in the sample is purified by n-pentanol extraction,and then reacts with azo reagents to generate orange compounds.The color depth of the solution is positively correlated with the content of histamine in the sample within a certain range.The histamine in the sample is qualitatively determined by visual colorimetry using the histamine standard color scale card.Result:The detection limit using sea bass,saury,and shrimp as blank matrices was 200 mg·kg-1,with a sensitivity and specificity of 100%and a false negative and false positive rate of 0%.Conclusion:This method is fast,accurate,highly sensitive,and suitable for the rapid detection of histamine in aquatic products.
关键词
组胺/水产品/方法学验证/快速检测Key words
histamine/aquatic products/methodological validation/rapid detection引用本文复制引用
基金项目
国家市场监督管理总局食品快检方法编制委托研究项目(KJ202102)
出版年
2024
NETL
NSTL
万方数据