Research on Rapid Detection of Histamine in Aquatic Products
Objective:To establish a rapid method for detecting histamine in aquatic products and validate it through methodological validation.Method:This study used the azo reagent colorimetric card method as a rapid detection method for histamine in aquatic products.After extraction,histamine in the sample is purified by n-pentanol extraction,and then reacts with azo reagents to generate orange compounds.The color depth of the solution is positively correlated with the content of histamine in the sample within a certain range.The histamine in the sample is qualitatively determined by visual colorimetry using the histamine standard color scale card.Result:The detection limit using sea bass,saury,and shrimp as blank matrices was 200 mg·kg-1,with a sensitivity and specificity of 100%and a false negative and false positive rate of 0%.Conclusion:This method is fast,accurate,highly sensitive,and suitable for the rapid detection of histamine in aquatic products.
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