Determination of Metronidazole and Dimetridazole Residues in Eggs by QuEChERS Combined with Ultra-High Performance Liquid Chromatography Tandem-Mass Spectrometry
A QuEChERS combined with ultra-high performance liquid chromatography tandem mass spectrometry method was established to determine the residues of metronidazole and dimetridazole in eggs.The sample was extracted with acetonitrile,purified with QuEChERS,and separated using an Agilent Eclipse Plus C18 column.The mobile phase was eluted with acetonitrile and 0.1%formic acid aqueous solution,and detected under multiple reaction monitoring mode.The external standard method was used for quantification.The results showed that the mass concentrations of metronidazole and dimetridazole were linear in the range of 0～50 ng·mL-1(r2＞0.999),and the detection limits were 0.002 μg·kg-1 and 0.007 μg·kg-1;under low,medium and high addition levels,the recoveries were 95.79%～97.80%,95.66%～100.10%respectively.The method is simple,reproducible and sensitive,and is suitable for the determination of metronidazole and dimetridazole in eggs.
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