QuEChERS-超高效液相色谱串联质谱法测定鸡蛋中甲硝唑和地美硝唑药物残留量
Determination of Metronidazole and Dimetridazole Residues in Eggs by QuEChERS Combined with Ultra-High Performance Liquid Chromatography Tandem-Mass Spectrometry
王易1
作者信息
- 1. 广东省惠州市质量计量监督检测所,广东惠州 516000
- 折叠
摘要
建立了QuEChERS结合超高效液相色谱串联质谱法测定鸡蛋中甲硝唑和地美硝唑残留量的方法.样品经乙腈提取,QuEChERS净化后,使用Agilent Eclipse Plus C18 柱分离,以乙腈和 0.1%甲酸水溶液为流动相进行洗脱,多反应监测模式下检测,外标法定量.结果表明,甲硝唑和地美硝唑的质量浓度在 0～50 ng·mL-1 时线性关系良好(r2＞0.999),检出限分别为 0.002 μg·kg-1、0.007 μg·kg-1;在低、中、高 3 个添加水平下甲硝唑和地美硝唑的加标回收率分别为95.79%～97.80%、95.66%～100.10%.该方法前处理简单、重现性好、灵敏度高,适用于鸡蛋中甲硝唑、地美硝唑的测定.
Abstract
A QuEChERS combined with ultra-high performance liquid chromatography tandem mass spectrometry method was established to determine the residues of metronidazole and dimetridazole in eggs.The sample was extracted with acetonitrile,purified with QuEChERS,and separated using an Agilent Eclipse Plus C18 column.The mobile phase was eluted with acetonitrile and 0.1%formic acid aqueous solution,and detected under multiple reaction monitoring mode.The external standard method was used for quantification.The results showed that the mass concentrations of metronidazole and dimetridazole were linear in the range of 0～50 ng·mL-1(r2＞0.999),and the detection limits were 0.002 μg·kg-1 and 0.007 μg·kg-1;under low,medium and high addition levels,the recoveries were 95.79%～97.80%,95.66%～100.10%respectively.The method is simple,reproducible and sensitive,and is suitable for the determination of metronidazole and dimetridazole in eggs.
关键词
鸡蛋/甲硝唑/地美硝唑/QuEChERS/超高效液相色谱串联质谱法Key words
eggs/metronidazole/dimetridazole/QuEChERS/ultra-high performance liquid chromatography tandem-mass spectrometry引用本文复制引用
出版年
2024
NETL
NSTL
万方数据