QuEChERS结合液相色谱质谱联用技术测定动物源性食品中N-二甲基亚硝胺
Determination of N-Dimethylnitrosamine in Food of Animal Origin by QuEChERS Combined with LC-MS/MS
赵广西 1刘志梅2
作者信息
- 1. 秦皇岛市食品药品检验中心,河北秦皇岛 066000
- 2. 秦皇岛市市场监督管理局,河北秦皇岛 066000
- 折叠
摘要
目的:建立高效液相色谱质谱联用技术定量检测动物源性食品中的N-二甲基亚硝胺.方法:样品前处理粉碎后经乙腈提取,低温离心除去油脂,QuEChERS净化,采用多反应监测模式测定.结果:N-二甲基亚硝胺校准曲线在 2.0～200.0 ng·mL-1 线性关系良好,相关系数为0.998 5,方法检出限和定量限分别为 0.5 μg·kg-1 和 3.0 μg·kg-1,回收率为 86.8%～90.8%,相对标准偏差在 2.2%～3.1%.结论:该方法取样量少,试剂消耗量少,提取和净化流程简便,定性和定量准确度好,可用于检测动源性食品中N-二甲基亚硝胺.
Abstract
Objective:To establish a high performance liquid chromatography-mass spectrometry method for the quantitative determination of N-dimethylnitrosamine in animal derived food.Method:The sample was extracted by acetonitrile after pretreatment and grinding,and the oil was removed by low temperature centrifugation,purified by QuEChERS,and determined by multi-reaction monitoring mode.Result:The calibration curve of N-dimethylnitrosamine had a good linear relationship in the range of 2.0～200.0 ng·mL-1,and the correlation coefficient was 0.998 5.The detection limit and quantitative limit of the method were 0.5 μg·kg-1 and 3.0 μg·kg-1,respectively.The recovery rate was 86.8%～90.8%,and the relative standard deviation was 2.2%～3.1%.Conclusion:This method has the advantages of less sampling,less reagent consumption,simple extraction and purif ication process,good qualitative and quantitative accuracy,and can be used to detect N-dimethylnitrosamine in animal-derived food.
关键词
高效液相色谱-质谱联用技术/QuEChERS/N-二甲基亚硝胺/多重反应监测Key words
high performance liquid chromatography-mass spectrometry/QuEChERS/N-dimethylnitrosamine/multiple reaction monitoring引用本文复制引用
出版年
2024
NETL
NSTL
万方数据