Determination of N-Dimethylnitrosamine in Food of Animal Origin by QuEChERS Combined with LC-MS/MS
Objective:To establish a high performance liquid chromatography-mass spectrometry method for the quantitative determination of N-dimethylnitrosamine in animal derived food.Method:The sample was extracted by acetonitrile after pretreatment and grinding,and the oil was removed by low temperature centrifugation,purified by QuEChERS,and determined by multi-reaction monitoring mode.Result:The calibration curve of N-dimethylnitrosamine had a good linear relationship in the range of 2.0～200.0 ng·mL-1,and the correlation coefficient was 0.998 5.The detection limit and quantitative limit of the method were 0.5 μg·kg-1 and 3.0 μg·kg-1,respectively.The recovery rate was 86.8%～90.8%,and the relative standard deviation was 2.2%～3.1%.Conclusion:This method has the advantages of less sampling,less reagent consumption,simple extraction and purif ication process,good qualitative and quantitative accuracy,and can be used to detect N-dimethylnitrosamine in animal-derived food.
high performance liquid chromatography-mass spectrometryQuEChERSN-dimethylnitrosaminemultiple reaction monitoring
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