高效液相色谱法测定芹菜中酚类化合物的含量
Determination of Phenolic Compounds in Celery by High Performance Liquid Chromatography
宫雪佳1
作者信息
- 1. 辽宁省食品检验检测院,辽宁沈阳 110000
- 折叠
摘要
采用高效液相色谱法测定芹菜样品中 11 种酚类化合物的含量.结果表明,11 种酚类化合物在 0.5～10.0 μg·mL-1 时,线性关系良好,相关系数均≥0.990,检出限为 0.07～0.29 mg·kg-1,定量限为0.20～0.84 mg·kg-1;在加标水平为 0.2 mg·kg-1、0.4 mg·kg-1 和 1.0 mg·kg-1 时,芹菜酚类化合物的回收率为71.90%～134.00%,相对标准偏差为 0.30%～4.00%,说明该方法具有较高的灵敏度和可靠性.
Abstract
Using high-performance liquid chromatography to determine the content of 11 phenolic compounds in celery samples.The results showed that 11 phenolic compounds ranged from 0.5 to 10.0 μg·mL-1,the linear relationship is good,with correlation coefficients≥0.990.The detection limit is 0.07～0.29 mg·kg-1,and the quantitative limit is 0.20～0.84 mg·kg-1.When the spiking levels were 0.2 mg·kg-1,0.4 mg·kg-1,and 1.0 mg·kg-1,the recovery rates of celery phenolic compounds were 71.90%～134.00%,and the relative standard deviation was 0.30%～4.00%,indicating that this method has high sensitivity and reliability.
关键词
高效液相色谱法/芹菜/酚类化合物Key words
high performance liquid chromatography/celery/phenolic compounds引用本文复制引用
出版年
2024
NETL
NSTL
万方数据