霉菌和酵母计数质控样品检验结果不确定度的评定
Evaluation of Uncertainty of Test Results of Quality Control Samples for Mold and Yeast Enumeration
曹克任 1孙荣嵘1
作者信息
- 1. 济南市食品药品检验检测中心,山东济南 250001
- 折叠
摘要
目的:评定质控样品中霉菌和酵母计数的不确定度.方法:根据《食品安全国家标准 食品微生物学检验 霉菌和酵母计数》(GB 4789.15-2016)和《食品微生物定量检测的测量不确定度评估指南》(RB/T 151-2016),采用平板计数法进行检验,分析不确定度分量来源并进行评定,进而合成检验结果的不确定度.结果:在置信概率 95%时,样品检验结果扩展不确定度为 0.040 9,检验结果区间为 3 700～4 500 CFU·mL-1.结论:影响检验结果不确定度的主要因素为1∶10样品匀液的制备和样品重复检测,因此实验室操作人员应加强相关因素的控制,提高检验结果的准确性.
Abstract
Objective:Evaluate the uncertainty of mold and yeast count test results of quality control samples.Method:According to the first method of GB 4789.15-2016 and RB/T 151-2016,plate counting was used methods are used to conduct inspections,the sources of uncertainty components are analyzed and evaluated,and then the uncertainty of the inspection results is synthesized.Result:When the confidence probability is 95%,the expanded uncertainty of the sample test results is 0.040 9,and the test result interval ranges from 3 700 to 4 500 CFU·mL-1.Conclusion:The main factors that affect the uncertainty of test results are the preparation of 1∶10 sample homogeneous solution and repeated testing of samples.Therefore,laboratory operators should strengthen the control of relevant factors to improve the accuracy of test results.
关键词
霉菌和酵母计数/不确定度/评定Key words
mold and yeast enumeration/uncertainty/evaluation引用本文复制引用
出版年
2024
NETL
NSTL
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