超高效液相色谱-串联质谱法同时测定水果中3种苯并咪唑类杀菌剂
Determination of Three Benzimidazole Fungicides in Fruits by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry
别怀周 1郑连港2
作者信息
- 1. 潍坊海润华辰检测技术有限公司,山东潍坊 261000
- 2. 山东世标检测技术有限公司,山东潍坊 261000
- 折叠
摘要
目的:构建一种超高效液相色谱-串联质谱法(Ultra Performance Liquid Chromatography Tandem Mass Spectrometry,UPLC-MS/MS)同时测定水果中3种苯并咪唑类杀菌剂的方法.方法:试样匀浆后用1%甲酸-乙腈超声提取,提取液经乙二胺-N-丙基硅烷和无水硫酸镁净化,采用UPLC-MS/MS测定.结果:3 种杀菌剂线性关系良好,相关系数R2 均大于 0.999,定量限在 0.05～1.16 μg·kg-1,选定 3 个浓度进行添加回收验证实验,回收率为 87.1%～103.2%,相对标准偏差为 1.4%～6.5%.结论:该方法简捷、准确,可满足水果中 3 种苯并咪唑杀菌剂的快速检测与确证分析要求.
Abstract
Objective:To develop a method for simultaneous determination of three benzimidazole fungicides in fruits by ultra-high performance liquid chromatography-tandem mass spectrometry.Method:The samples were homogenized,followed by ultrasonic extraction with 1%formic acid-acetonitrile,the extraction solution was purified by primary secondary amine and anhydrous magnesium sulfate,and detected by UPLC-MS/MS.Result:The linearity of each fungicide was good,with linear correlation coefficients were greater than 0.999,and the limits of detection were 0.05～1.16 μg·kg-1,three concentrations were selected for the addition and recovery experiment,and the recovery rate of spiked samples were 87.1%～103.2%,and the relative standard deviations were 1.4%～6.5%.Conclusion:The method is simple,convenient and accurate,and is suitable for the rapid detection and confirmatory analysis of three benzimidazole fungicides in fruits.
关键词
苯并咪唑类杀菌剂/水果/超高效液相色谱-串联质谱法Key words
benzimidazole fungicides/fruit/ultra performance liquid chromatography tandem mass spectrometry引用本文复制引用
出版年
2024
NETL
NSTL
万方数据