In recent years,the phenomenon of excessive levels of Pseudomonas aeruginosa in packaged drinking water has occurred.The use of national safety standard methods for inspection is cumbersome and time-consuming,which is not conducive to supervision.However,the real-time fluorescence PCR detection method has the advantages of sensitivity,speed,and accuracy,and is widely used in various testing methods.In this paper,real-time fluorescent PCR was used to amplify the target gene with specific primers and probes.Then,the other 5 kinds of bacteria and the gradient content of Pseudomonas aeruginosa suspensions were detected by real-time fluorescent PCR to verify the specificity and sensitivity of the method.The results showed that only Pseudomonas aeruginosa was positively detected by real-time fluorescent PCR,while the detection results of other bacteria were negative,indicating that the specificity of this method for Pseudomonas aeruginosa was good,and the sensitivity of this method was 1×103 CFU·mL-1.Compared with the national safety standard method,the real-time fluorescent PCR method takes less time and significantly improves the detection efficiency.
关键词
铜绿假单胞菌/实时荧光PCR/包装饮用水
Key words
Pseudomonas aeruginosa/real-time fluorescent quantitative PCR/packaged drinking water
维普
万方数据