Study on the Detection of Pseudomonas aeruginosa in Packaged Drinking Water by Real-Time Fluorescent PCR
In recent years,the phenomenon of excessive levels of Pseudomonas aeruginosa in packaged drinking water has occurred.The use of national safety standard methods for inspection is cumbersome and time-consuming,which is not conducive to supervision.However,the real-time fluorescence PCR detection method has the advantages of sensitivity,speed,and accuracy,and is widely used in various testing methods.In this paper,real-time fluorescent PCR was used to amplify the target gene with specific primers and probes.Then,the other 5 kinds of bacteria and the gradient content of Pseudomonas aeruginosa suspensions were detected by real-time fluorescent PCR to verify the specificity and sensitivity of the method.The results showed that only Pseudomonas aeruginosa was positively detected by real-time fluorescent PCR,while the detection results of other bacteria were negative,indicating that the specificity of this method for Pseudomonas aeruginosa was good,and the sensitivity of this method was 1×103 CFU·mL-1.Compared with the national safety standard method,the real-time fluorescent PCR method takes less time and significantly improves the detection efficiency.
Pseudomonas aeruginosareal-time fluorescent quantitative PCRpackaged drinking water
万方数据
维普
