Determination of Deoxynivalenol in Wheat Flour by Immunoaffinity Chromatography High Performance Liquid Chromatography
Objective:To establish an immunoaffinity chromatography method for the determination of deoxynivalenol in wheat flour.Method:Wheat flour was crushed and extracted with water.After immunoaffinity column sampling,purification and elution,the content of deoxynivalenol in wheat flour was determined by HPLC.Result:In the range of 100～5 000 ng·mL-1,the linearity of the method was good,the correlation coefficient(R2)was 0.999 8,the detection limit was 48 ng·mL-1,the recovery was 90.1%～109.6%,and the relative standard deviation was 1.49%～2.20%.Conclusion:The method is simple,specific and precise,and can be used as a treatment scheme for mass analysis of deoxynivalenol content in wheat flour.
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