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富硒葛根粉中葛根素含量的测定方法

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目的:采用HPLC和LC-MS相结合的方法,建立了富硒葛根粉中葛根素含量的测定方法.方法:样品经 70%乙醇提取,利用岛津Inertsil ODS-SP C18 柱(250 mm×4.6 mm,5 µm)分离,以甲醇-0.3%磷酸(20∶80)为流动相,测定富硒葛根粉中葛根素含量.结果:在0.1～1.3 µg·mL-1,葛根素的线性关系良好,平均加标回收率为97.39%,RSD为 1.08%.结论:HPLC法简便、快速,重复性好,可作为富硒葛根粉中葛根素含量的测定方法.
Method for Determination of Puerarin Content in Selenium-Enriched Pueraria Powder
Objective:To establish a method for the determination of puerarin in selenium-enriched pueraria powder by HPLC and LC-MS.Method:The samples were extracted by 70%ethanol and the content of puerarin in selene-enriched pueraria powder was determined by using Shimadzu Inertsil ODS-SPC18 column(250 mm×4.6 mm,5 µm)with methanol-0.3%phosphoric acid(20 ∶ 80)as mobile phase.Result:In the range of 0.1～1.3 µg·mL-1,the linear relationship of puerarin was good,the average recovery rate was 97.39%,RSD was 1.08%.Conclusion:HPLC is a simple,rapid and reproducible method for the determination of puerarin in selenium-enriched pueraria powder.

selenium-enriched pueraria powderpuerarincontent determination

龙泽霞、杨芳

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湖南省常德市食品检验所,湖南常德 415000

富硒葛根粉 葛根素 含量测定

湖南省市场监督管理局科技项目(2023)

2024

食品安全导刊
商业科技质量中心 北京肉类食品协会

食品安全导刊

影响因子：0.065
ISSN：1674-0270
年,卷(期)：2024.(7)
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